PURPOSE: This study investigates the effect of cell seeding density on cartilage repair in matrix-assisted chondrocyte implantation in vitro and in vivo. METHODS: In vitro: Four different cell seeding densities of human chondrocytes were seeded onto a porous methoxy-polyethylene glycol-polylactic-co-glycolic acid scaffold (MPEG-PLGA) polymer scaffold ASEED™ (1.2 × 10(6), 4.0 × 10(6), 1.2 × 10(7) and 2.0 × 10(7) cells/cm(3)). The cartilage repair response was evaluated by relative gene expression of the chondrogenic markers sox9, collagen types I, II and X, and aggrecan, total DNA content and sulphated glycosaminoglycan synthesis. In vivo: Using a New Zealand white rabbit intercondylar osteochondral defect model, three different cell seeding densities (1.2 × 10(6), 4.0 × 10(6) and 1.2 × 10(7) cells/cm(3)) were tested with an empty scaffold as control. The cartilage repair response was evaluated using O'Driscoll score. RESULTS: In vitro: A significant difference (p < 0.05) in total DNA content was found at day 2 but not at day 7. The low cell seeding densities yielded the highest GAG content (p < 0.001) at day 7. Collagen type I was highest (p < 0.01) at the lowest density at day 7. In vivo: No significant difference was found between the 4 groups. CONCLUSIONS: No positive effect on cartilage repair was found using increased cell seeding density. LEVEL OF EVIDENCE: Controlled experimental study, Level II.
PURPOSE: This study investigates the effect of cell seeding density on cartilage repair in matrix-assisted chondrocyte implantation in vitro and in vivo. METHODS: In vitro: Four different cell seeding densities of human chondrocytes were seeded onto a porous methoxy-polyethylene glycol-polylactic-co-glycolic acid scaffold (MPEG-PLGA) polymer scaffold ASEED™ (1.2 × 10(6), 4.0 × 10(6), 1.2 × 10(7) and 2.0 × 10(7) cells/cm(3)). The cartilage repair response was evaluated by relative gene expression of the chondrogenic markers sox9, collagen types I, II and X, and aggrecan, total DNA content and sulphated glycosaminoglycan synthesis. In vivo: Using a New Zealand white rabbit intercondylar osteochondral defect model, three different cell seeding densities (1.2 × 10(6), 4.0 × 10(6) and 1.2 × 10(7) cells/cm(3)) were tested with an empty scaffold as control. The cartilage repair response was evaluated using O'Driscoll score. RESULTS: In vitro: A significant difference (p < 0.05) in total DNA content was found at day 2 but not at day 7. The low cell seeding densities yielded the highest GAG content (p < 0.001) at day 7. Collagen type I was highest (p < 0.01) at the lowest density at day 7. In vivo: No significant difference was found between the 4 groups. CONCLUSIONS: No positive effect on cartilage repair was found using increased cell seeding density. LEVEL OF EVIDENCE: Controlled experimental study, Level II.
Authors: Silvia E Francioli; Christian Candrian; Katja Martin; Michael Heberer; Ivan Martin; Andrea Barbero Journal: J Biomed Mater Res A Date: 2010-12-01 Impact factor: 4.396
Authors: Mohammed A Yassin; Knut N Leknes; Torbjorn O Pedersen; Zhe Xing; Yang Sun; Stein A Lie; Anna Finne-Wistrand; Kamal Mustafa Journal: J Biomed Mater Res A Date: 2015-09-04 Impact factor: 4.396
Authors: Troy D Bornes; Nadr M Jomha; Aillette Mulet-Sierra; Adetola B Adesida Journal: Tissue Eng Part C Methods Date: 2016-01-18 Impact factor: 3.056
Authors: Sarah E Bulman; Cynthia M Coleman; J Mary Murphy; Nicholas Medcalf; Aideen E Ryan; Frank Barry Journal: Stem Cell Res Ther Date: 2015-03-19 Impact factor: 6.832
Authors: Claire Mennan; John Garcia; Helen McCarthy; Sharon Owen; Jade Perry; Karina Wright; Robin Banerjee; James B Richardson; Sally Roberts Journal: Cartilage Date: 2018-04-19 Impact factor: 4.634
Authors: Vivian H M Mouser; Noël M M Dautzenberg; Riccardo Levato; Mattie H P van Rijen; Wouter J A Dhert; Jos Malda; Debby Gawlitta Journal: ALTEX Date: 2017-09-08 Impact factor: 6.043