Literature DB >> 22485921

SDS-Polyacrylamide Gel Electrophoresis of Proteins.

Joseph Sambrook, David W Russell.   

Abstract

INTRODUCTIONThis protocol describes the separation of proteins by SDS-polyacrylamide gel electrophoresis. SDS is used with a reducing agent and heat to dissociate the proteins. SDS-polypeptide complexes form and migrate through the gels according to the size of the polypeptide. By using markers of known molecular weight, the molecular weight of the polypeptide chain(s) can be estimated.

Entities:  

Year:  2006        PMID： 22485921     DOI： 10.1101/pdb.prot4540

Source DB:  PubMed          Journal:  CSH Protoc        ISSN： 1559-6095


  24 in total

1.  Glucose delays the insulin-induced increase in thyroid hormone-mediated signaling in adipose of prolong-fasted elephant seal pups.

Authors:  Bridget Martinez; José G Soñanez-Organis; Jose A Viscarra; John T Jaques; Duncan S MacKenzie; Daniel E Crocker; Rudy M Ortiz
Journal:  Am J Physiol Regul Integr Comp Physiol       Date:  2016-01-06       Impact factor: 3.619

2.  A tightly regulated and adjustable CRISPR-dCas9 based AND gate in yeast.

Authors:  Anja Hofmann; Johannes Falk; Tim Prangemeier; Dominic Happel; Adrian Köber; Andreas Christmann; Heinz Koeppl; Harald Kolmar
Journal:  Nucleic Acids Res       Date:  2019-01-10       Impact factor: 16.971

3.  Construction and expression of a novel anti-CD14 human-mouse chimeric antibody Hm2F9.

Authors:  Di-Ying Shen; Bo-Tao Ning; Yong-Min Tang; Si-Si Li
Journal:  DNA Cell Biol       Date:  2014-06-06       Impact factor: 3.311

4.  Quantitative assessment of RNA-protein interactions with high-throughput sequencing-RNA affinity profiling.

Authors:  Abdullah Ozer; Jacob M Tome; Robin C Friedman; Dan Gheba; Gary P Schroth; John T Lis
Journal:  Nat Protoc       Date:  2015-07-16       Impact factor: 13.491

5.  Analysis of Protein Stability by Synthesis Shutoff.

Authors:  Ira Buntenbroich; Tânia Simões; Mafalda Escobar-Henriques
Journal:  Bio Protoc       Date:  2021-11-20

6.  Respiratory Selenite Reductase from Bacillus selenitireducens Strain MLS10.

Authors:  Michael Wells; Jennifer McGarry; Maissa M Gaye; Partha Basu; Ronald S Oremland; John F Stolz
Journal:  J Bacteriol       Date:  2019-03-13       Impact factor: 3.490

7.  Prolonged food deprivation increases mRNA expression of deiodinase 1 and 2, and thyroid hormone receptor β-1 in a fasting-adapted mammal.

Authors:  Bridget Martinez; José G Soñanez-Organis; José Pablo Vázquez-Medina; Jose A Viscarra; Duncan S MacKenzie; Daniel E Crocker; Rudy M Ortiz
Journal:  J Exp Biol       Date:  2013-12-15       Impact factor: 3.312

8.  A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions.

Authors:  Tabitha A Peterson; Mark A Stamnes; Robert C Piper
Journal:  J Vis Exp       Date:  2018-06-06       Impact factor: 1.355

9.  The Acetyltransferase RibT From Bacillus subtilis Affects in vivo Dynamics of the Multimeric Heavy Riboflavin Synthase Complex.

Authors:  Daniel Andreas Orlando Rotter; Christoph Heger; Christian Kühm; Nina Schmidt; Antje Schäfer; Thomas Heimerl; Matthias Mack; Peter L Graumann
Journal:  Front Microbiol       Date:  2022-04-14       Impact factor: 5.640

10.  A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters.

Authors:  Wenyuan Gao; Kai Wu; Lifeng Chen; Haiyang Fan; Zhiqiang Zhao; Bei Gao; Hualei Wang; Dongzhi Wei
Journal:  Microb Cell Fact       Date:  2016-02-18       Impact factor: 5.328
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