| Literature DB >> 22484851 |
Julia Ladewig1, Jerome Mertens, Jaideep Kesavan, Jonas Doerr, Daniel Poppe, Finnja Glaue, Stefan Herms, Peter Wernet, Gesine Kögler, Franz-Josef Müller, Philipp Koch, Oliver Brüstle.
Abstract
Forced expression of proneural transcription factors has been shown to direct neuronal conversion of fibroblasts. Because neurons are postmitotic, conversion efficiencies are an important parameter for this process. We present a minimalist approach combining two-factor neuronal programming with small molecule-based inhibition of glycogen synthase kinase-3β and SMAD signaling, which converts postnatal human fibroblasts into functional neuron-like cells with yields up to >200% and neuronal purities up to >80%.Entities:
Mesh：
Substances：
Year: 2012 PMID： 22484851 DOI： 10.1038/nmeth.1972
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547