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Literature DB >> 22484846

Derivation of iPSCs in stirred suspension bioreactors.

Mehdi Shafa¹, Brad Day, Akihiro Yamashita, Guoliang Meng, Shiying Liu, Roman Krawetz, Derrick E Rancourt.

Abstract

Induced pluripotent stem cells (iPSCs) are typically derived in adherent culture. Here we report fast and efficient derivation of mouse iPSCs in stirred suspension bioreactors, with and without the use of c-Myc. Suspension-reprogrammed cells expressed pluripotency markers, showed multilineage differentiation in vitro and in vivo, and contributed to the germline in chimeric mice. Suspension reprogramming has the potential to accelerate and standardize iPSC research.

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Year: 2012 PMID： 22484846 DOI： 10.1038/nmeth.1973

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

12 in total

1. Embryonic stem cells remain highly pluripotent following long term expansion as aggregates in suspension bioreactors.

Authors: Nicole I zur Nieden; Jaymi T Cormier; Derrick E Rancourt; Michael S Kallos
Journal: J Biotechnol Date: 2007-01-21 Impact factor: 3.307

2. Expansion of undifferentiated murine embryonic stem cells as aggregates in suspension culture bioreactors.

Authors: Jaymi T Cormier; Nicole I zur Nieden; Derrick E Rancourt; Michael S Kallos
Journal: Tissue Eng Date: 2006-11

Review 3. The systematic production of cells for cell therapies.

Authors: Daniel C Kirouac; Peter W Zandstra
Journal: Cell Stem Cell Date: 2008-10-09 Impact factor: 24.633

4. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.

Authors: Kazutoshi Takahashi; Shinya Yamanaka
Journal: Cell Date: 2006-08-10 Impact factor: 41.582

5. Expansion of mammalian neural stem cells in bioreactors: effect of power input and medium viscosity.

Authors: Arindom Sen; Michael S Kallos; Leo A Behie
Journal: Brain Res Dev Brain Res Date: 2002-03-31

6. Promotion of direct reprogramming by transformation-deficient Myc.

Authors: Masato Nakagawa; Nanako Takizawa; Megumi Narita; Tomoko Ichisaka; Shinya Yamanaka
Journal: Proc Natl Acad Sci U S A Date: 2010-07-26 Impact factor: 11.205

7. Reduced differentiation efficiency of murine embryonic stem cells in stirred suspension bioreactors.

Authors: Jaymi T Taiani; Roman J Krawetz; Nicole I Zur Nieden; Yiru Elizabeth Wu; Michael S Kallos; John R Matyas; Derrick E Rancourt
Journal: Stem Cells Dev Date: 2010-07 Impact factor: 3.272

8. Generation of germline-competent induced pluripotent stem cells.

Authors: Keisuke Okita; Tomoko Ichisaka; Shinya Yamanaka
Journal: Nature Date: 2007-06-06 Impact factor: 49.962

9. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts.

Authors: Masato Nakagawa; Michiyo Koyanagi; Koji Tanabe; Kazutoshi Takahashi; Tomoko Ichisaka; Takashi Aoi; Keisuke Okita; Yuji Mochiduki; Nanako Takizawa; Shinya Yamanaka
Journal: Nat Biotechnol Date: 2007-11-30 Impact factor: 54.908

10. piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells.

Authors: Knut Woltjen; Iacovos P Michael; Paria Mohseni; Ridham Desai; Maria Mileikovsky; Riikka Hämäläinen; Rebecca Cowling; Wei Wang; Pentao Liu; Marina Gertsenstein; Keisuke Kaji; Hoon-Ki Sung; Andras Nagy
Journal: Nature Date: 2009-03-01 Impact factor: 49.962

17 in total

1. Reprogramming in suspension.

Authors: Jiekai Chen; Duanqing Pei
Journal: Nat Methods Date: 2012-04-27 Impact factor: 28.547

2. Scalable culture of human pluripotent stem cells in 3D.

Authors: Todd C McDevitt
Journal: Proc Natl Acad Sci U S A Date: 2013-12-10 Impact factor: 11.205

3. A highly optimized protocol for reprogramming cancer cells to pluripotency using nonviral plasmid vectors.

Authors: Hongzhi Zhao; Timothy J Davies; Jiaolin Ning; Yanxu Chang; Patty Sachamitr; Susanne Sattler; Paul J Fairchild; Fang-Ping Huang
Journal: Cell Reprogram Date: 2014-12-30 Impact factor: 1.987

4. One-step microfluidic generation of pre-hatching embryo-like core-shell microcapsules for miniaturized 3D culture of pluripotent stem cells.

Authors: Pranay Agarwal; Shuting Zhao; Peter Bielecki; Wei Rao; Jung Kyu Choi; Yi Zhao; Jianhua Yu; Wujie Zhang; Xiaoming He
Journal: Lab Chip Date: 2013-12-07 Impact factor: 6.799

Review 5. Induced pluripotent stem cells for regenerative medicine.

Authors: Karen K Hirschi; Song Li; Krishnendu Roy
Journal: Annu Rev Biomed Eng Date: 2014-05-29 Impact factor: 9.590

6. A massive suspension culture system with metabolic purification for human pluripotent stem cell-derived cardiomyocytes.

Authors: Natsuko Hemmi; Shugo Tohyama; Kazuaki Nakajima; Hideaki Kanazawa; Tomoyuki Suzuki; Fumiyuki Hattori; Tomohisa Seki; Yoshikazu Kishino; Akinori Hirano; Marina Okada; Ryota Tabei; Rei Ohno; Chihana Fujita; Tomoko Haruna; Shinsuke Yuasa; Motoaki Sano; Jun Fujita; Keiichi Fukuda
Journal: Stem Cells Transl Med Date: 2014-10-29 Impact factor: 6.940

7. Biophysical regulation of cell reprogramming.

Authors: Sze Yue Wong; Jennifer Soto; Song Li
Journal: Curr Opin Chem Eng Date: 2017-02 Impact factor: 5.163

Review 8. Bioreactor engineering of stem cell environments.

Authors: Nina Tandon; Darja Marolt; Elisa Cimetta; Gordana Vunjak-Novakovic
Journal: Biotechnol Adv Date: 2013-03-24 Impact factor: 14.227

9. Coaxial electrospray of liquid core-hydrogel shell microcapsules for encapsulation and miniaturized 3D culture of pluripotent stem cells.

Authors: Shuting Zhao; Pranay Agarwal; Wei Rao; Haishui Huang; Renliang Zhang; Zhenguo Liu; Jianhua Yu; Noah Weisleder; Wujie Zhang; Xiaoming He
Journal: Integr Biol (Camb) Date: 2014-09 Impact factor: 2.192

Review 10. Hydrodynamic modulation of pluripotent stem cells.

Authors: Krista M Fridley; Melissa A Kinney; Todd C McDevitt
Journal: Stem Cell Res Ther Date: 2012-11-20 Impact factor: 6.832