OBJECTIVE: To better understand possible mechanisms involved in the dysregulation of gene expression unique to oral squamous cell carcinoma (OSCC) metastasis, the investigators examined the differential expression of microRNAs (miRNAs) in OSCC metastasis and their functional impact on target gene expression. STUDY DESIGN: Observational assessment of DNA copy number, miRNA, and RNA expression in primary and metastatic OSCC. SETTING: University of Washington Medical Center and affiliated hospitals. SUBJECTS: Tumor samples were taken from patients with primary incident OSCC; cells were laser-capture microdissected from 17 nonmetastatic primary tumors and 20 metastatic lymph nodes. METHODS: DNA copy number aberrations and gene expression profiles were previously determined using Affymetrix 250K Nsp I SNP arrays and HU133 plus 2.0 expression arrays. miRNAs were interrogated with Exiqon's Ready-to-Use PCR Panels assessing the expression of 368 human miRNAs. RESULTS: Investigators found 31 miRNAs differentially expressed between metastatic and nonmetastatic samples (false discovery rate <0.4; 26 overexpressed and 5 underexpressed in metastatic samples). Expression of 7 of these miRNAs was significantly associated with their DNA copy numbers, and expressions of 8 of these miRNAs were significantly associated with their target genes. Among these unique miRNAs, miR-140-3p, miR-29c, and miR-29a were differentially expressed in metastasis versus nonmetastatic samples and had a strong positive correlation with their DNA copy numbers and a negative correlation with the expression of their target genes. CONCLUSION: Results suggest that DNA copy number aberration may play a role in the dysregulation of some differentially expressed miRNAs in OSCC metastasis, warranting further investigation.
OBJECTIVE: To better understand possible mechanisms involved in the dysregulation of gene expression unique to oral squamous cell carcinoma (OSCC) metastasis, the investigators examined the differential expression of microRNAs (miRNAs) in OSCC metastasis and their functional impact on target gene expression. STUDY DESIGN: Observational assessment of DNA copy number, miRNA, and RNA expression in primary and metastatic OSCC. SETTING: University of Washington Medical Center and affiliated hospitals. SUBJECTS:Tumor samples were taken from patients with primary incident OSCC; cells were laser-capture microdissected from 17 nonmetastatic primary tumors and 20 metastatic lymph nodes. METHODS: DNA copy number aberrations and gene expression profiles were previously determined using Affymetrix 250K Nsp I SNP arrays and HU133 plus 2.0 expression arrays. miRNAs were interrogated with Exiqon's Ready-to-Use PCR Panels assessing the expression of 368 human miRNAs. RESULTS: Investigators found 31 miRNAs differentially expressed between metastatic and nonmetastatic samples (false discovery rate <0.4; 26 overexpressed and 5 underexpressed in metastatic samples). Expression of 7 of these miRNAs was significantly associated with their DNA copy numbers, and expressions of 8 of these miRNAs were significantly associated with their target genes. Among these unique miRNAs, miR-140-3p, miR-29c, and miR-29a were differentially expressed in metastasis versus nonmetastatic samples and had a strong positive correlation with their DNA copy numbers and a negative correlation with the expression of their target genes. CONCLUSION: Results suggest that DNA copy number aberration may play a role in the dysregulation of some differentially expressed miRNAs in OSCC metastasis, warranting further investigation.
Authors: Latha Ramdas; Uma Giri; Cheryl L Ashorn; Kevin R Coombes; Adel El-Naggar; K Kian Ang; Michael D Story Journal: Head Neck Date: 2009-05 Impact factor: 3.147
Authors: E Bandrés; E Cubedo; X Agirre; R Malumbres; R Zárate; N Ramirez; A Abajo; A Navarro; I Moreno; M Monzó; J García-Foncillas Journal: Mol Cancer Date: 2006-07-19 Impact factor: 27.401
Authors: C B Lajer; F C Nielsen; L Friis-Hansen; B Norrild; R Borup; E Garnæs; M Rossing; L Specht; M H Therkildsen; B Nauntofte; S Dabelsteen; C von Buchwald Journal: Br J Cancer Date: 2011-02-15 Impact factor: 7.640
Authors: William Warnica; Daniele Merico; Gregory Costain; Simon E Alfred; John Wei; Christian R Marshall; Stephen W Scherer; Anne S Bassett Journal: Biol Psychiatry Date: 2014-05-29 Impact factor: 13.382
Authors: Camile B Lopes; Leandro L Magalhães; Carolina R Teófilo; Ana Paula N N Alves; Raquel C Montenegro; Massimo Negrini; Ândrea Ribeiro-Dos-Santos Journal: BMC Cancer Date: 2018-07-06 Impact factor: 4.430
Authors: Mohd Younis Bhat; Jayshree Advani; Pavithra Rajagopalan; Krishna Patel; Vishalakshi Nanjappa; Hitendra S Solanki; Arun H Patil; Firdous A Bhat; Premendu P Mathur; Bipin Nair; T S Keshava Prasad; Joseph A Califano; David Sidransky; Harsha Gowda; Aditi Chatterjee Journal: Sci Rep Date: 2018-05-04 Impact factor: 4.379