BACKGROUND: The expression of miRNA in head and neck squamous cell carcinomas (HNSCCs) that had been classified as high risk by surgical pathologic features and validated by trial outcome for disease recurrence was determined and compared with matched adjacent normal tissues. METHODS: miRNA and corresponding gene expression were determined using miRNA bioarrays and gene expression arrays. RESULTS: Twenty miRNAs were determined to be differentially regulated in the HNSCC samples when compared with their normal tissue counterparts. Quantitative reverse transcriptase-polymerase chain reaction confirmed differential regulation of miRNA expression, and gene expression analysis on these same-paired samples confirmed the loss of putative mRNA targets including genes such as adenomatous polyposis coli, programmed cell death protein 4, and TGF beta receptor 3 in the tumor samples. CONCLUSIONS: These data suggest a role for the upregulation of specific miRNAs in high-risk HNSCC. Furthermore, upregulation of these miRNAs may be responsible for the elimination of mRNAs that lead to the growth and progression of HNSCC.
BACKGROUND: The expression of miRNA in head and neck squamous cell carcinomas (HNSCCs) that had been classified as high risk by surgical pathologic features and validated by trial outcome for disease recurrence was determined and compared with matched adjacent normal tissues. METHODS: miRNA and corresponding gene expression were determined using miRNA bioarrays and gene expression arrays. RESULTS: Twenty miRNAs were determined to be differentially regulated in the HNSCC samples when compared with their normal tissue counterparts. Quantitative reverse transcriptase-polymerase chain reaction confirmed differential regulation of miRNA expression, and gene expression analysis on these same-paired samples confirmed the loss of putative mRNA targets including genes such as adenomatous polyposis coli, programmed cell death protein 4, and TGF beta receptor 3 in the tumor samples. CONCLUSIONS: These data suggest a role for the upregulation of specific miRNAs in high-risk HNSCC. Furthermore, upregulation of these miRNAs may be responsible for the elimination of mRNAs that lead to the growth and progression of HNSCC.
Authors: Azra Krek; Dominic Grün; Matthew N Poy; Rachel Wolf; Lauren Rosenberg; Eric J Epstein; Philip MacMenamin; Isabelle da Piedade; Kristin C Gunsalus; Markus Stoffel; Nikolaus Rajewsky Journal: Nat Genet Date: 2005-04-03 Impact factor: 38.330
Authors: Huiling He; Krystian Jazdzewski; Wei Li; Sandya Liyanarachchi; Rebecca Nagy; Stefano Volinia; George A Calin; Chang-Gong Liu; Kaarle Franssila; Saul Suster; Richard T Kloos; Carlo M Croce; Albert de la Chapelle Journal: Proc Natl Acad Sci U S A Date: 2005-12-19 Impact factor: 11.205
Authors: Peggy S Eis; Wayne Tam; Liping Sun; Amy Chadburn; Zongdong Li; Mario F Gomez; Elsebet Lund; James E Dahlberg Journal: Proc Natl Acad Sci U S A Date: 2005-02-28 Impact factor: 11.205
Authors: Jun Lu; Gad Getz; Eric A Miska; Ezequiel Alvarez-Saavedra; Justin Lamb; David Peck; Alejandro Sweet-Cordero; Benjamin L Ebert; Raymond H Mak; Adolfo A Ferrando; James R Downing; Tyler Jacks; H Robert Horvitz; Todd R Golub Journal: Nature Date: 2005-06-09 Impact factor: 49.962
Authors: Joost Kluiver; Eugenia Haralambieva; Debora de Jong; Tjasso Blokzijl; Susan Jacobs; Bart-Jan Kroesen; Sibrand Poppema; Anke van den Berg Journal: Genes Chromosomes Cancer Date: 2006-02 Impact factor: 5.006
Authors: Marilena V Iorio; Manuela Ferracin; Chang-Gong Liu; Angelo Veronese; Riccardo Spizzo; Silvia Sabbioni; Eros Magri; Massimo Pedriali; Muller Fabbri; Manuela Campiglio; Sylvie Ménard; Juan P Palazzo; Anne Rosenberg; Piero Musiani; Stefano Volinia; Italo Nenci; George A Calin; Patrizia Querzoli; Massimo Negrini; Carlo M Croce Journal: Cancer Res Date: 2005-08-15 Impact factor: 12.701
Authors: Christine H Chung; Joel S Parker; Gamze Karaca; Junyuan Wu; William K Funkhouser; Dominic Moore; Dale Butterfoss; Dong Xiang; Adam Zanation; Xiaoying Yin; William W Shockley; Mark C Weissler; Lynn G Dressler; Carol G Shores; Wendell G Yarbrough; Charles M Perou Journal: Cancer Cell Date: 2004-05 Impact factor: 31.743
Authors: Bino John; Anton J Enright; Alexei Aravin; Thomas Tuschl; Chris Sander; Debora S Marks Journal: PLoS Biol Date: 2004-10-05 Impact factor: 8.029
Authors: Angela J Yoon; Shuang Wang; Jing Shen; Nicolas Robine; Elizabeth Philipone; Martin W Oster; Albert Nam; Regina M Santella Journal: Am J Transl Res Date: 2014-10-11 Impact factor: 4.060
Authors: Brock C Christensen; Michele Avissar-Whiting; Lauren G Ouellet; Rondi A Butler; Heather H Nelson; Michael D McClean; Carmen J Marsit; Karl T Kelsey Journal: Clin Cancer Res Date: 2010-05-25 Impact factor: 12.531
Authors: Alexandra Lucs; Benjamin Saltman; Christine H Chung; Bettie M Steinberg; David L Schwartz Journal: Head Neck Date: 2012-01-27 Impact factor: 3.147
Authors: Nicholas A Serrano; Chang Xu; Yan Liu; Pei Wang; Wenhong Fan; Melissa P Upton; John R Houck; Pawadee Lohavanichbutr; Michael Kao; Lue Ping Zhao; Stephen M Schwartz; Chu Chen; Eduardo Méndez Journal: Otolaryngol Head Neck Surg Date: 2012-04-02 Impact factor: 3.497
Authors: Yitan Zhu; Abdallah S R Mohamed; Stephen Y Lai; Shengjie Yang; Aasheesh Kanwar; Lin Wei; Mona Kamal; Subhajit Sengupta; Hesham Elhalawani; Heath Skinner; Dennis S Mackin; Jay Shiao; Jay Messer; Andrew Wong; Yao Ding; Lifei Zhang; Laurence Court; Yuan Ji; Clifton D Fuller Journal: JCO Clin Cancer Inform Date: 2019-02
Authors: Brock C Christensen; Benjamin J Moyer; Michele Avissar; Lauren G Ouellet; Silvia L Plaza; Michael D McClean; Carmen J Marsit; Karl T Kelsey Journal: Carcinogenesis Date: 2009-04-20 Impact factor: 4.944