Literature DB >> 22465282

2D gels and their third-dimension potential.

Jorge B Schvartzman1, María-Luisa Martínez-Robles, Virginia López, Pablo Hernández, Dora B Krimer.   

Abstract

Two-dimensional (2D) agarose gel electrophoresis is one of the most powerful methods to analyze the mass and shape of replication intermediates. It is often use to map replication origins but it is also useful to characterize termination of replication, replication fork barriers and even replication fork reversal. Here, we present protocols, figures and movies with a thorough description of different modes of replication for linear DNA fragments and the corresponding patterns they generate in 2D gels.
Copyright © 2012 Elsevier Inc. All rights reserved.

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Year:  2012        PMID： 22465282     DOI： 10.1016/j.ymeth.2012.03.013

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  8 in total

Review 1.  Replication fork reversal in eukaryotes: from dead end to dynamic response.

Authors:  Kai J Neelsen; Massimo Lopes
Journal:  Nat Rev Mol Cell Biol       Date:  2015-02-25       Impact factor: 94.444

Review 2.  Thymineless death, at the origin.

Authors:  Elena C Guzmán; Carmen M Martín
Journal:  Front Microbiol       Date:  2015-05-19       Impact factor: 5.640

3.  Topoisomerase 2 is dispensable for the replication and segregation of small yeast artificial chromosomes (YACs).

Authors:  Jorge Cebrián; Estefanía Monturus; María-Luisa Martínez-Robles; Pablo Hernández; Dora B Krimer; Jorge B Schvartzman
Journal:  PLoS One       Date:  2014-08-12       Impact factor: 3.240

4.  Electrophoretic mobility of supercoiled, catenated and knotted DNA molecules.

Authors:  Jorge Cebrián; Maridian J Kadomatsu-Hermosa; Alicia Castán; Víctor Martínez; Cristina Parra; María José Fernández-Nestosa; Christian Schaerer; María-Luisa Martínez-Robles; Pablo Hernández; Dora B Krimer; Andrzej Stasiak; Jorge B Schvartzman
Journal:  Nucleic Acids Res       Date:  2014-11-20       Impact factor: 16.971

5.  Analysis of DNA topology of EBV minichromosomes in HEK 293 cells.

Authors:  Alicia Castán; Vanessa Fernández-Calleja; Pablo Hernández; Dora B Krimer; Jorge B Schvartzman; María-José Fernández-Nestosa
Journal:  PLoS One       Date:  2017-11-29       Impact factor: 3.240

6.  Differential requirement of Srs2 helicase and Rad51 displacement activities in replication of hairpin-forming CAG/CTG repeats.

Authors:  Jennifer H G Nguyen; David Viterbo; Ranjith P Anand; Lauren Verra; Laura Sloan; Guy-Franck Richard; Catherine H Freudenreich
Journal:  Nucleic Acids Res       Date:  2017-05-05       Impact factor: 16.971

Review 7.  Closing the DNA replication cycle: from simple circular molecules to supercoiled and knotted DNA catenanes.

Authors:  Jorge B Schvartzman; Pablo Hernández; Dora B Krimer; Julien Dorier; Andrzej Stasiak
Journal:  Nucleic Acids Res       Date:  2019-08-22       Impact factor: 16.971

8.  The abundance of Fob1 modulates the efficiency of rRFBs to stall replication forks.

Authors:  Alicia Castán; Pablo Hernández; Dora B Krimer; Jorge B Schvartzman
Journal:  Nucleic Acids Res       Date:  2017-09-29       Impact factor: 16.971
  8 in total

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