Literature DB >> 22461279

Genetically encoded pH sensor for tracking surface proteins through endocytosis.

Anmol Grover1, Brigitte F Schmidt, Russell D Salter, Simon C Watkins, Alan S Waggoner, Marcel P Bruchez.   

Abstract

Traffic cam: a tandem dye prepared from a FRET acceptor and a fluorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization serves to follow protein trafficking during endocytosis. This sensor was used to analyze agonist-dependent internalization of β(2)-adrenergic receptors. It was also used as a surrogate antigen to reveal direct surface-to-endosome antigen transfer between dendritic cells (not shown).
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2012        PMID: 22461279      PMCID: PMC3538816          DOI: 10.1002/anie.201108107

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN: 1433-7851            Impact factor:   15.336


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