| Literature DB >> 22444666 |
M E Ali1, U Hashim, S Mustafa, Y B Che Man, Th S Dhahi, M Kashif, Md Kamal Uddin, S B Abd Hamid.
Abstract
A test for assessing pork adulteration in meatballs, using TaqMan probe real-time polymerase chain reaction, was developed. The assay combined porcine-specific primers and TaqMan probe for the detection of a 109 bp fragment of porcine cytochrome b gene. Specificity test with 10 ng DNA of eleven different species yielded a threshold cycle (Ct) of 15.5 ± 0.20 for the pork and negative results for the others. Analysis of beef meatballs with spiked pork showed the assay can determine 100-0.01% contaminated pork with 102% PCR efficiency, high linear regression (r(2) = 0.994) and ≤ 6% relative errors. Residuals analysis revealed a high precision in all determinations. Random analysis of commercial meatballs from pork, beef, chicken, mutton and goat, yielded a Ct between 15.89 ± 0.16 and 16.37 ± 0.22 from pork meatballs and negative results from the others, showing the suitability of the assay to determine pork in commercial meatballs with a high accuracy and precision.Entities:
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Year: 2012 PMID： 22444666 DOI： 10.1016/j.meatsci.2012.02.031
Source DB: PubMed Journal: Meat Sci ISSN： 0309-1740 Impact factor: 5.209