Literature DB >> 22441391

Analysis of defined combinations of monoclonal antibodies in anthrax toxin neutralization assays and their synergistic action.

Miriam M Ngundi1, Bruce D Meade, Stephen F Little, Conrad P Quinn, Cindi R Corbett, Rebecca A Brady, Drusilla L Burns.   

Abstract

Antibodies against the protective antigen (PA) component of anthrax toxin play an important role in protection against disease caused by Bacillus anthracis. In this study, we examined defined combinations of PA-specific monoclonal antibodies for their ability to neutralize anthrax toxin in cell culture assays. We observed additive, synergistic, and antagonistic effects of the antibodies depending on the specific antibody combination examined and the specific assay used. Synergistic toxin-neutralizing antibody interactions were examined in more detail. We found that one mechanism that can lead to antibody synergy is the bridging of PA monomers by one antibody, with resultant bivalent binding of the second antibody. These results may aid in optimal design of new vaccines and antibody therapies against anthrax.

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Year:  2012        PMID： 22441391      PMCID： PMC3346336          DOI： 10.1128/CVI.05714-11

Source DB:  PubMed          Journal:  Clin Vaccine Immunol        ISSN： 1556-679X


  42 in total

1.  Search for correlates of protective immunity conferred by anthrax vaccine.

Authors:  S Reuveny; M D White; Y Y Adar; Y Kafri; Z Altboum; Y Gozes; D Kobiler; A Shafferman; B Velan
Journal:  Infect Immun       Date:  2001-05       Impact factor: 3.441

2.  Contribution of lethal toxin and edema toxin to the pathogenesis of anthrax meningitis.

Authors:  Celia M Ebrahimi; Tamsin R Sheen; Christian W Renken; Roberta A Gottlieb; Kelly S Doran
Journal:  Infect Immun       Date:  2011-04-25       Impact factor: 3.441

3.  The role of antibodies to Bacillus anthracis and anthrax toxin components in inhibiting the early stages of infection by anthrax spores.

Authors:  S Welkos; S Little; A Friedlander; D Fritz; P Fellows
Journal:  Microbiology       Date:  2001-06       Impact factor: 2.777

4.  In vitro correlate of immunity in a rabbit model of inhalational anthrax.

Authors:  M L Pitt; S F Little; B E Ivins; P Fellows; J Barth; J Hewetson; P Gibbs; M Dertzbaugh; A M Friedlander
Journal:  Vaccine       Date:  2001-09-14       Impact factor: 3.641

5.  Protection against anthrax toxin by recombinant antibody fragments correlates with antigen affinity.

Authors:  Jennifer A Maynard; Catharina B M Maassen; Stephen H Leppla; Kathleen Brasky; Jean L Patterson; Brent L Iverson; George Georgiou
Journal:  Nat Biotechnol       Date:  2002-06       Impact factor: 54.908

6.  Potent neutralization of botulinum neurotoxin by recombinant oligoclonal antibody.

Authors:  A Nowakowski; C Wang; D B Powers; P Amersdorfer; T J Smith; V A Montgomery; R Sheridan; R Blake; L A Smith; J D Marks
Journal:  Proc Natl Acad Sci U S A       Date:  2002-08-12       Impact factor: 11.205

7.  Defining a serological correlate of protection in rabbits for a recombinant anthrax vaccine.

Authors:  S F Little; B E Ivins; P F Fellows; M L M Pitt; S L W Norris; G P Andrews
Journal:  Vaccine       Date:  2004-01-02       Impact factor: 3.641

Review 8.  Anthrax toxin.

Authors:  R John Collier; John A T Young
Journal:  Annu Rev Cell Dev Biol       Date:  2003       Impact factor: 13.827

9.  Cutting edge: anthrax lethal toxin inhibits activation of IFN-regulatory factor 3 by lipopolysaccharide.

Authors:  Oanh Dang; Lorena Navarro; Keith Anderson; Michael David
Journal:  J Immunol       Date:  2004-01-15       Impact factor: 5.422

Review 10.  The roles of anthrax toxin in pathogenesis.

Authors:  Mahtab Moayeri; Stephen H Leppla
Journal:  Curr Opin Microbiol       Date:  2004-02       Impact factor: 7.934
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  10 in total

1.  Complex mixtures of antibodies generated from a single production qualitatively and quantitatively evaluated by native Orbitrap mass spectrometry.

Authors:  Natalie J Thompson; Linda J A Hendriks; John de Kruif; Mark Throsby; Albert J R Heck
Journal:  MAbs       Date:  2014 Jan-Feb       Impact factor: 5.857

2.  Broadly neutralizing anti-influenza virus antibodies: enhancement of neutralizing potency in polyclonal mixtures and IgA backbones.

Authors:  Wenqian He; Caitlin E Mullarkey; J Andrew Duty; Thomas M Moran; Peter Palese; Matthew S Miller
Journal:  J Virol       Date:  2015-01-14       Impact factor: 5.103

3.  Synergistic Neutralization of Pertussis Toxin by a Bispecific Antibody In Vitro and In Vivo.

Authors:  Ellen K Wagner; Xianzhe Wang; Andre Bui; Jennifer A Maynard
Journal:  Clin Vaccine Immunol       Date:  2016-11-04

4.  Accurate and selective quantification of anthrax protective antigen in plasma by immunocapture and isotope dilution mass spectrometry.

Authors:  Maria I Solano; Adrian R Woolfitt; Anne E Boyer; Renato C Lins; Katie Isbell; Maribel Gallegos-Candela; Hercules Moura; Carrie L Pierce; John R Barr
Journal:  Analyst       Date:  2019-03-25       Impact factor: 4.616

5.  Disease-enhancing antibodies improve the efficacy of bacterial toxin-neutralizing antibodies.

Authors:  Siu-Kei Chow; Cameron Smith; Thomas MacCarthy; Mary Ann Pohl; Aviv Bergman; Arturo Casadevall
Journal:  Cell Host Microbe       Date:  2013-04-17       Impact factor: 21.023

6.  Combinations of monoclonal antibodies to anthrax toxin manifest new properties in neutralization assays.

Authors:  Mary Ann Pohl; Johanna Rivera; Antonio Nakouzi; Siu-Kei Chow; Arturo Casadevall
Journal:  Infect Immun       Date:  2013-03-18       Impact factor: 3.441

Review 7.  Monoclonal Antibody Combinations that Present Synergistic Neutralizing Activity: A Platform for Next-Generation Anti-Toxin Drugs.

Authors:  Eran Diamant; Amram Torgeman; Eyal Ozeri; Ran Zichel
Journal:  Toxins (Basel)       Date:  2015-05-29       Impact factor: 4.546

8.  A Novel Chimeric Anti-PA Neutralizing Antibody for Postexposure Prophylaxis and Treatment of Anthrax.

Authors:  Siping Xiong; Qi Tang; Xudong Liang; Tingting Zhou; Jin Yang; Peng Liu; Ya Chen; Changjun Wang; Zhenqing Feng; Jin Zhu
Journal:  Sci Rep       Date:  2015-07-02       Impact factor: 4.379

9.  Quantitative Determination of Lethal Toxin Proteins in Culture Supernatant of Human Live Anthrax Vaccine Bacillus anthracis A16R.

Authors:  Xiaodong Zai; Jun Zhang; Ju Liu; Jie Liu; Liangliang Li; Ying Yin; Ling Fu; Junjie Xu; Wei Chen
Journal:  Toxins (Basel)       Date:  2016-02-25       Impact factor: 4.546

10.  Immunological Evidence of Variation in Exposure and Immune Response to Bacillus anthracis in Herbivores of Kruger and Etosha National Parks.

Authors:  Sunday O Ochai; Jan E Crafford; Ayesha Hassim; Charles Byaruhanga; Yen-Hua Huang; Axel Hartmann; Edgar H Dekker; O Louis van Schalkwyk; Pauline L Kamath; Wendy C Turner; Henriette van Heerden
Journal:  Front Immunol       Date:  2022-02-14       Impact factor: 7.561
  10 in total

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