Literature DB >> 22434781

A universal expression tag for structural and functional studies of proteins.

Vladimir V Rogov1, Alexis Rozenknop, Natalia Yu Rogova, Frank Löhr, Suhas Tikole, Victor Jaravine, Peter Güntert, Ivan Dikic, Volker Dötsch.   

Abstract

Modified ubiquitin sequences, each completed with a His tag and a TEV cleavage site, were designed to enhance the expression of protein/peptide targets. With this new system we have been able to characterize several peptide-protein interactions by ITC and by NMR and CD spectroscopic methods, including the interactions of LIR domains with autophagy modifiers.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2012        PMID： 22434781     DOI： 10.1002/cbic.201200045

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  20 in total

1.  Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold.

Authors:  Adam Norek; Lubomír Janda
Journal:  Protein Sci       Date:  2017-02-11       Impact factor: 6.725

2.  The three-dimensional structure of an H-superfamily conotoxin reveals a granulin fold arising from a common ICK cysteine framework.

Authors:  Lau D Nielsen; Mads M Foged; Anastasia Albert; Andreas B Bertelsen; Cecilie L Søltoft; Samuel D Robinson; Steen V Petersen; Anthony W Purcell; Baldomero M Olivera; Raymond S Norton; Terje Vasskog; Helena Safavi-Hemami; Kaare Teilum; Lars Ellgaard
Journal:  J Biol Chem       Date:  2019-04-11       Impact factor: 5.157

3.  Structural and Functional Analysis of a Novel Interaction Motif within UFM1-activating Enzyme 5 (UBA5) Required for Binding to Ubiquitin-like Proteins and Ufmylation.

Authors:  Sabrina Habisov; Jessica Huber; Yoshinobu Ichimura; Masato Akutsu; Natalia Rogova; Frank Loehr; David G McEwan; Terje Johansen; Ivan Dikic; Volker Doetsch; Masaaki Komatsu; Vladimir V Rogov; Vladimir Kirkin
Journal:  J Biol Chem       Date:  2016-02-29       Impact factor: 5.157

4.  Role of SH3b binding domain in a natural deletion mutant of Kayvirus endolysin LysF1 with a broad range of lytic activity.

Authors:  Martin Benešík; Jiří Nováček; Lubomír Janda; Radka Dopitová; Markéta Pernisová; Kateřina Melková; Lenka Tišáková; Jiří Doškař; Lukáš Žídek; Jan Hejátko; Roman Pantůček
Journal:  Virus Genes       Date:  2017-08-29       Impact factor: 2.332

5.  Site-specific inhibition of the small ubiquitin-like modifier (SUMO)-conjugating enzyme Ubc9 selectively impairs SUMO chain formation.

Authors:  Svenja Wiechmann; Anne Gärtner; Andreas Kniss; Andreas Stengl; Christian Behrends; Vladimir V Rogov; Manuel S Rodriguez; Volker Dötsch; Stefan Müller; Andreas Ernst
Journal:  J Biol Chem       Date:  2017-08-07       Impact factor: 5.157

6.  Oxygen-dependent asparagine hydroxylation of the ubiquitin-associated (UBA) domain in Cezanne regulates ubiquitin binding.

Authors:  Julia Mader; Jessica Huber; Florian Bonn; Volker Dötsch; Vladimir V Rogov; Anja Bremm
Journal:  J Biol Chem       Date:  2020-01-14       Impact factor: 5.157

7.  Disassembly of Lys11 and mixed linkage polyubiquitin conjugates provides insights into function of proteasomal deubiquitinases Rpn11 and Ubp6.

Authors:  Wissam Mansour; Mark A Nakasone; Maximilian von Delbrück; Zanlin Yu; Daria Krutauz; Noa Reis; Oded Kleifeld; Thomas Sommer; David Fushman; Michael H Glickman
Journal:  J Biol Chem       Date:  2014-11-11       Impact factor: 5.157

8.  An atypical LIR motif within UBA5 (ubiquitin like modifier activating enzyme 5) interacts with GABARAP proteins and mediates membrane localization of UBA5.

Authors:  Jessica Huber; Miki Obata; Jens Gruber; Masato Akutsu; Frank Löhr; Natalia Rogova; Peter Güntert; Ivan Dikic; Vladimir Kirkin; Masaaki Komatsu; Volker Dötsch; Vladimir V Rogov
Journal:  Autophagy       Date:  2019-04-28       Impact factor: 16.016

9.  Fluorescence-based ATG8 sensors monitor localization and function of LC3/GABARAP proteins.

Authors:  Alexandra Stolz; Mateusz Putyrski; Ivana Kutle; Jessica Huber; Chunxin Wang; Viktória Major; Sachdev S Sidhu; Richard J Youle; Vladimir V Rogov; Volker Dötsch; Andreas Ernst; Ivan Dikic
Journal:  EMBO J       Date:  2016-12-27       Impact factor: 11.598

10.  Intermediate filament-associated cytolinker plectin 1c destabilizes microtubules in keratinocytes.

Authors:  Rocio G Valencia; Gernot Walko; Lubomir Janda; Jirka Novacek; Eva Mihailovska; Siegfried Reipert; Kerstin Andrä-Marobela; Gerhard Wiche
Journal:  Mol Biol Cell       Date:  2013-01-30       Impact factor: 4.138
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