BACKGROUND: It is common practice during one lung ventilation (OLV) to use 100% oxygen, although this may cause hyperoxia- and oxidative stress-related lung injury. We hypothesized that lower oxygen (FiO(2) ) during OLV will result in less inflammatory and oxidative lung injury and improved lung function. METHODS: Twenty pigs (8.88 ± 0.84 kg; 38 ± 4.6 days) were assigned to either the hyperoxia group (n = 10; FiO(2) = 100%) or the normoxia group (n = 10; FiO(2) < 50%). Both groups were subjected to 3 hr of OLV. Blood samples were tested for pro-inflammatory cytokines and lung tissue was tested for these cytokines and oxidative biomarkers. RESULTS: There were no differences between groups for partial pressure of CO(2) , tidal volume, end-tidal CO(2) , plasma cytokines, or respiratory compliance. Total respiratory resistance was greater in the hyperoxia group (P = 0.02). There were higher levels of TNF-α, IL-1β, and IL-6 in the lung homogenates of the hyperoxia group than in the normoxia group (P ≤ 0.01, 0.001, and 0.001, respectively). Myeloperoxidase and protein carbonyls (PC) were higher (P = 0.03 and P = 0.01, respectively) and superoxide dismutase (SOD) was lower in the lung homogenates of the hyperoxia group (P ≤ 0.001). CONCLUSION: Higher myeloperoxidase, PC, and cytokine levels, and lower SOD availability indicate a greater degree of injury in the lungs of the hyperoxia animals, possibly from using 100% oxygen. In this translational study using a pig model, FiO(2) ≤ 50% during OLV reduced hyperoxic injury and improved function in the lungs.
BACKGROUND: It is common practice during one lung ventilation (OLV) to use 100% oxygen, although this may cause hyperoxia- and oxidative stress-related lung injury. We hypothesized that lower oxygen (FiO(2) ) during OLV will result in less inflammatory and oxidative lung injury and improved lung function. METHODS: Twenty pigs (8.88 ± 0.84 kg; 38 ± 4.6 days) were assigned to either the hyperoxia group (n = 10; FiO(2) = 100%) or the normoxia group (n = 10; FiO(2) < 50%). Both groups were subjected to 3 hr of OLV. Blood samples were tested for pro-inflammatory cytokines and lung tissue was tested for these cytokines and oxidative biomarkers. RESULTS: There were no differences between groups for partial pressure of CO(2) , tidal volume, end-tidal CO(2) , plasma cytokines, or respiratory compliance. Total respiratory resistance was greater in the hyperoxia group (P = 0.02). There were higher levels of TNF-α, IL-1β, and IL-6 in the lung homogenates of the hyperoxia group than in the normoxia group (P ≤ 0.01, 0.001, and 0.001, respectively). Myeloperoxidase and protein carbonyls (PC) were higher (P = 0.03 and P = 0.01, respectively) and superoxide dismutase (SOD) was lower in the lung homogenates of the hyperoxia group (P ≤ 0.001). CONCLUSION: Higher myeloperoxidase, PC, and cytokine levels, and lower SOD availability indicate a greater degree of injury in the lungs of the hyperoxia animals, possibly from using 100% oxygen. In this translational study using a pig model, FiO(2) ≤ 50% during OLV reduced hyperoxic injury and improved function in the lungs.
Authors: Stephan Christen; Barbara Finckh; Jens Lykkesfeldt; Peter Gessler; Manuela Frese-Schaper; Peter Nielsen; Edith R Schmid; Bernhard Schmitt Journal: Free Radic Biol Med Date: 2005-05-15 Impact factor: 7.376
Authors: Vineet Bhandari; Rayman Choo-Wing; Chun G Lee; Zhou Zhu; Jonathan H Nedrelow; Geoffrey L Chupp; Xucher Zhang; Michael A Matthay; Lorraine B Ware; Robert J Homer; Patty J Lee; Anke Geick; Antonin R de Fougerolles; Jack A Elias Journal: Nat Med Date: 2006-11-05 Impact factor: 53.440
Authors: Thomas L Miller; Clifford J Singhaus; Tami I Sherman; Jay S Greenspan; Thomas H Shaffer Journal: Pediatr Crit Care Med Date: 2006-03 Impact factor: 3.624
Authors: Mary C Theroux; Alicia Olivant Fisher; Maria E Rodriguez; Robert P Brislin; Kirk W Reichard; Suken A Shah; Matt McCoy; Melinda Brown; Kirk W Dabney; William G Mackenzie; Douglas A Katz; Thomas H Shaffer Journal: Paediatr Anaesth Date: 2015-01-02 Impact factor: 2.556