PURPOSE: DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. METHODS: A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment. RESULTS: The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P < 0.05), oligozoospermic (P < 0.001) and teratozoospermic samples (P < 0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable. CONCLUSIONS: Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.
PURPOSE: DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. METHODS: A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment. RESULTS: The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P < 0.05), oligozoospermic (P < 0.001) and teratozoospermic samples (P < 0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable. CONCLUSIONS: Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.
Authors: M Kheirollahi-Kouhestani; S Razavi; M Tavalaee; M R Deemeh; M Mardani; J Moshtaghian; M H Nasr-Esfahani Journal: Hum Reprod Date: 2009-06-24 Impact factor: 6.918
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Authors: Jacob Netherton; Rachel A Ogle; Louise Hetherington; Ana Izabel Silva Balbin Villaverde; Hubert Hondermarck; Mark A Baker Journal: Mol Cell Proteomics Date: 2019-12-17 Impact factor: 5.911
Authors: Hamilton De Martin; Marcello S Cocuzza; Bruno C Tiseo; Guilherme J A Wood; Eduardo P Miranda; Pedro A A Monteleone; José Maria Soares; Paulo C Serafini; Miguel Srougi; Edmund C Baracat Journal: J Assist Reprod Genet Date: 2017-09-19 Impact factor: 3.412