| Literature DB >> 22397740 |
Patricia Soares Wyant1, Stephan Strohmeier, Benjamin Schäfer, Björn Krenz, Iraildes Pereira Assunção, Gaus Silvestre de Andrade Lima, Holger Jeske.
Abstract
Circomics was coined to describe the combination of rolling circle amplification (RCA), restriction fragment length polymorphism (RFLP) and pyro-sequencing to investigate the genome structures of small circular DNAs. A batch procedure is described using 61 plant samples from Asia, South America and Central America which revealed 83 contig sequences of geminiviral DNA components and 4 contig sequences of DNA satellites. The usefulness of this approach is validated for the Brazilian begomoviruses, and the sequence fidelity is determined by comparing the results with those of conventional cloning and sequencing of Bolivian begomoviruses reported recently. Therefore, circomics has been proven to be a major step forward to economize costs and labor and to characterize reliably geminiviral genomes in their population structure of the quasispecies. Copyright ÂEntities:
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Year: 2012 PMID： 22397740 DOI： 10.1016/j.virol.2012.02.007
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616