Hamid Alizadeh Nili1, Hossein Mozdarani1, Franck Pellestor2. 1. Dept. of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, P.O. Box: 14115-111, Tehran, Iran. 2. INSERM U847, IRB, Hôpital ST Eloi, 34295 Montpellier cedex 5, France.
Abstract
BACKGROUND: Various frequencies of sperm aneuploidy are reported in sperms of subfertile patients compared to normal individuals. Moreover, sperm DNA damage is shown to be associated with male infertility. In this study, the rate of DNA damage and frequencies of aneuploidy in sperms of subfertile patients was investigated. METHODS: Semen samples were obtained from healthy normal and subfertile (oligozoospermia, asthenozoospermia, and oligoasthenozoospermia) men. The frequency of aneuploidy was assessed using primed in situ labeling (PRINS) analysis with specific primers for chromosomes 18, 21, X, and Y. Sperm DNA damage was assessed using alkaline comet assay. RESULTS: The mean frequencies of disomy for the patients were significantly higher than normal for all chromosomes (P < 0.01). The extent of DNA damage in sperms of subfertiles was significantly higher than in normal individuals (P < 0.001). The obtained results indicated that higher rate of DNA damages led to higher frequency of chromosomal disomy except for asthenozoospermia samples which exhibited higher rate of DNA damage and lower frequency of chromosomal disomy. CONCLUSIONS: These results demonstrate that men with oligozoospermia and oligoasthenozoospermia have an elevated risk for chromosome abnormalities in their sperm, particularly sex chromosomes. DNA damage might be involved in the process of malsegregation of chromosomes.
BACKGROUND: Various frequencies of sperm aneuploidy are reported in sperms of subfertile patients compared to normal individuals. Moreover, sperm DNA damage is shown to be associated with male infertility. In this study, the rate of DNA damage and frequencies of aneuploidy in sperms of subfertile patients was investigated. METHODS: Semen samples were obtained from healthy normal and subfertile (oligozoospermia, asthenozoospermia, and oligoasthenozoospermia) men. The frequency of aneuploidy was assessed using primed in situ labeling (PRINS) analysis with specific primers for chromosomes 18, 21, X, and Y. Sperm DNA damage was assessed using alkaline comet assay. RESULTS: The mean frequencies of disomy for the patients were significantly higher than normal for all chromosomes (P < 0.01). The extent of DNA damage in sperms of subfertiles was significantly higher than in normal individuals (P < 0.001). The obtained results indicated that higher rate of DNA damages led to higher frequency of chromosomal disomy except for asthenozoospermia samples which exhibited higher rate of DNA damage and lower frequency of chromosomal disomy. CONCLUSIONS: These results demonstrate that men with oligozoospermia and oligoasthenozoospermia have an elevated risk for chromosome abnormalities in their sperm, particularly sex chromosomes. DNA damage might be involved in the process of malsegregation of chromosomes.
Entities:
Keywords:
Asthenozoospermia; DNA damage, Primed in situ labeling; Comet assay
Authors: Gordon Kirkpatrick; Kyle A Ferguson; Haijun Gao; Steven Tang; Victor Chow; Basil Ho Yuen; Sai Ma Journal: Hum Reprod Date: 2008-04-24 Impact factor: 6.918