| Literature DB >> 22385080 |
Qi Tong, Xin Wang, Hui Wang, Takuya Kubo, Mingdi Yan.
Abstract
We report a new type of microarray, based on glyconanoparticles (GNPs), to study glycan-lectin interactions. GNPs, synthesized by conjugating carbohydrate ligands on silica nanoparticles, were printed on a photoactive surface followed by covalent immobilization by light activation. The GNP microarrays could be probed by lectins labeled with fluorescein as well as fluorescein-doped silica nanoparticles (FSNPs). Results showed that FSNP as the label enhanced the signals for the higher affinity ligands than the lower ones.Entities:
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Year: 2012 PMID: 22385080 PMCID: PMC3405919 DOI: 10.1021/ac203455b
Source DB: PubMed Journal: Anal Chem ISSN: 0003-2700 Impact factor: 6.986