OBJECTIVES: Aim of this study was to evaluate the accuracy and precision of the detection of individual miRNA as clinical biomarkers in the serum. DESIGN AND METHODS: miRNA-126 was quantified in serum using endogenous and exogenous controls for normalization and the accuracy and precision of the method evaluated. The diagnostic value of serum miRNA-126 was evaluated in malignant mesothelioma (MM) and non-small-cell lung cancer (NSCLC) patients using both relative and absolute qRT-PCR methods. RESULTS: The use of endogenous invariant and exogenous synthetic controls as well sample dilution markedly improves the accuracy and precision of the assay. The inter- and intra-assay analyses revealed that relative qRT-PCR is a more reliable method. Circulating miR-126 detected in the serum by relative qRT-PCRs was found low-expressed in both malignancies, significantly differentiated MM patients from healthy controls and NSCLC from MM, but do not discriminate NSCLC patients from control subjects. Kaplan-Meier analysis revealed that low level of circulating miR-126 in MM patients was strongly associated with worse prognosis. CONCLUSIONS: We propose that this approach can be adopted for accurate analysis of other suitable circulating miRNA markers of different types of cancer.
OBJECTIVES: Aim of this study was to evaluate the accuracy and precision of the detection of individual miRNA as clinical biomarkers in the serum. DESIGN AND METHODS: miRNA-126 was quantified in serum using endogenous and exogenous controls for normalization and the accuracy and precision of the method evaluated. The diagnostic value of serum miRNA-126 was evaluated in malignant mesothelioma (MM) and non-small-cell lung cancer (NSCLC) patients using both relative and absolute qRT-PCR methods. RESULTS: The use of endogenous invariant and exogenous synthetic controls as well sample dilution markedly improves the accuracy and precision of the assay. The inter- and intra-assay analyses revealed that relative qRT-PCR is a more reliable method. Circulating miR-126 detected in the serum by relative qRT-PCRs was found low-expressed in both malignancies, significantly differentiated MMpatients from healthy controls and NSCLC from MM, but do not discriminate NSCLCpatients from control subjects. Kaplan-Meier analysis revealed that low level of circulating miR-126 in MMpatients was strongly associated with worse prognosis. CONCLUSIONS: We propose that this approach can be adopted for accurate analysis of other suitable circulating miRNA markers of different types of cancer.
Authors: A Truini; S Coco; A Alama; C Genova; C Sini; M G Dal Bello; G Barletta; E Rijavec; G Burrafato; F Boccardo; F Grossi Journal: Cell Mol Life Sci Date: 2014-02-23 Impact factor: 9.261
Authors: Marco Tomasetti; Linda Nocchi; Sara Staffolani; Nicola Manzella; Monica Amati; Jacob Goodwin; Katarina Kluckova; Maria Nguyen; Elisabetta Strafella; Martina Bajzikova; Martin Peterka; Sandra Lettlova; Jaroslav Truksa; Wan Lee; Lan-Feng Dong; Lory Santarelli; Jiri Neuzil Journal: Antioxid Redox Signal Date: 2014-04-23 Impact factor: 8.401
Authors: Alberto Izzotti; Stefano Carozzo; Alessandra Pulliero; Dinara Zhabayeva; Jean Louis Ravetti; Rakhmet Bersimbaev Journal: Am J Cancer Res Date: 2016-07-01 Impact factor: 6.166