CONTEXT: Adipose tissue is one of the first organs to develop insulin resistance even with moderate BMI. However, the contribution of developing hyperglycaemia and concomitant methylglyoxal increment to tissue dysfunction during type 2 diabetes progression was not addressed before. METHODS: Young and aged Wistar and Goto-Kakizaki rats (non-obese model of type 2 diabetes) and a group of MG-treated W rats were used to investigate the chronic effects of hyperglycaemia and ageing and specifically MG-induced mechanisms. RESULTS: Diabetic and aged rats showed decreased adipose tissue irrigation and interstitial hypoxia. Hyperglycaemia of diabetic rats leaded to fibrosis and accumulation of PAS-positive components, exacerbated in aged animals, which also showed decreased hipoadiponectinemia, increased MCP-1 expression and macrophage infiltration to glycated fibrotic regions. MG leaded to increased free fatty acids, hipoadiponectinemia, decreased irrigation, hypoxia and macrophage recruitment for glycated fibrotic regions. CONCLUSIONS: MG contributes to dysfunction of adipose tissue during type 2 diabetes progression.
CONTEXT: Adipose tissue is one of the first organs to develop insulin resistance even with moderate BMI. However, the contribution of developing hyperglycaemia and concomitant methylglyoxal increment to tissue dysfunction during type 2 diabetes progression was not addressed before. METHODS: Young and aged Wistar and Goto-Kakizaki rats (non-obese model of type 2 diabetes) and a group of MG-treated W rats were used to investigate the chronic effects of hyperglycaemia and ageing and specifically MG-induced mechanisms. RESULTS:Diabetic and aged rats showed decreased adipose tissue irrigation and interstitial hypoxia. Hyperglycaemia of diabeticrats leaded to fibrosis and accumulation of PAS-positive components, exacerbated in aged animals, which also showed decreased hipoadiponectinemia, increased MCP-1 expression and macrophage infiltration to glycated fibrotic regions. MG leaded to increased free fatty acids, hipoadiponectinemia, decreased irrigation, hypoxia and macrophage recruitment for glycated fibrotic regions. CONCLUSIONS:MG contributes to dysfunction of adipose tissue during type 2 diabetes progression.
Authors: Hana Malínská; Olena Oliyarnyk; Vojtěch Škop; Jan Šilhavý; Vladimír Landa; Václav Zídek; Petr Mlejnek; Miroslava Šimáková; Hynek Strnad; Ludmila Kazdová; Michal Pravenec Journal: PLoS One Date: 2016-03-10 Impact factor: 3.240
Authors: Tamaeh Monteiro-Alfredo; Paulo Matafome; Bianca Pancoti Iacia; Kátia Ávila Antunes; Jéssica Maurino Dos Santos; Janielle da Silva Melo da Cunha; Sara Oliveira; Alex Santos Oliveira; Jaqueline Ferreira Campos; Mariana Magalhães; Célia Cabral; Raquel Seiça; Cláudia Andrea Lima Cardoso; Caio Fernando Ramalho de Oliveira; Edson Lucas Dos Santos; Kely de Picoli Souza Journal: Oxid Med Cell Longev Date: 2020-03-22 Impact factor: 6.543
Authors: Irena Markova; Martina Hüttl; Olena Oliyarnyk; Tereza Kacerova; Martin Haluzik; Petr Kacer; Ondrej Seda; Hana Malinska Journal: Nutr Metab (Lond) Date: 2019-08-01 Impact factor: 4.169