Literature DB >> 22359166

The usefulness of CHAPS as a non-cytotoxic stabilizing agent in purification of growth factors.

Y Matuo1, N Nishi, Y Muguruma, Y Yoshitake, Y Masuda, K Nishikawa, F Wada.   

Abstract

Among several detergents, a zwitterionic detergent, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHAPS), was found to be least cytotoxic for cultured mammalian cells. CHAPS improved the activity recovery and elution profile of crude and purified fibroblast growth factors (FGFs) during chromatographies. Diluted preparations of FGFs were stabilized by CHAPS against the loss during storage. Amino acid sequence analysis was not disturbed by CHAPS. CHAPS was removable by reversed-phase high-performance liquid chromatography. These results indicate that CHAPS is useful as a non-cytotoxic stabilizing agent in purification of various kinds of bioactive polypeptides.

Entities:  

Year:  1988        PMID： 22359166     DOI： 10.1007/BF00365076

Source DB:  PubMed          Journal:  Cytotechnology        ISSN： 0920-9069            Impact factor:   2.058


  26 in total

1.  The use of zwitterionic surfactants in the agarose chromatography of biological membranes.

Authors:  J C Allen; C Humphries
Journal:  FEBS Lett       Date:  1975-09-15       Impact factor: 4.124

2.  Direct mitogenic effects of insulin, epidermal growth factor, glucocorticoid, cholera toxin, unknown pituitary factors and possibly prolactin, but not androgen, on normal rat prostate epithelial cells in serum-free, primary cell culture.

Authors:  W L McKeehan; P S Adams; M P Rosser
Journal:  Cancer Res       Date:  1984-05       Impact factor: 12.701

3.  Solubilization of membrane proteins by sulfobetaines, novel zwitterionic surfactants.

Authors:  A Gonenne; R Ernst
Journal:  Anal Biochem       Date:  1978-06-15       Impact factor: 3.365

4.  A nondenaturing zwitterionic detergent for membrane biochemistry: design and synthesis.

Authors:  L M Hjelmeland
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

5.  Electrofocusing of integral membrane proteins in mixtures of zwitterionic and nonionic detergents.

Authors:  L M Hjelmeland; D W Nebert; A Chrambach
Journal:  Anal Biochem       Date:  1979-05       Impact factor: 3.365

6.  High and low affinity binding sites for basic fibroblast growth factor on cultured cells: absence of a role for low affinity binding in the stimulation of plasminogen activator production by bovine capillary endothelial cells.

Authors:  D Moscatelli
Journal:  J Cell Physiol       Date:  1987-04       Impact factor: 6.384

7.  Solubilized active pituitary and ovarian gonadotropin-releasing hormone receptors retain binding properties for adenosine 3',5'-cyclic monophosphate derivatives.

Authors:  A M Capponi; M L Aubert; R N Clayton
Journal:  Life Sci       Date:  1984-05-28       Impact factor: 5.037

8.  Heparin protects basic and acidic FGF from inactivation.

Authors:  D Gospodarowicz; J Cheng
Journal:  J Cell Physiol       Date:  1986-09       Impact factor: 6.384

9.  Solubilization and purification of rat liver 5'-nucleotidase by use of a zwitterionic detergent and a monoclonal-antibody immunoadsorbent.

Authors:  E M Bailyes; A C Newby; K Siddle; J P Luzio
Journal:  Biochem J       Date:  1982-04-01       Impact factor: 3.857

10.  Detergent solubilization of the interleukin 1 receptor.

Authors:  K A Paganelli; A S Stern; P L Kilian
Journal:  J Immunol       Date:  1987-04-01       Impact factor: 5.422
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  2 in total

1.  Distribution of fibroblast growth factors in cultured tumor cells and their transplants.

Authors:  Y Yoshitake; K Nishikawa
Journal:  In Vitro Cell Dev Biol       Date:  1992-06

2.  Production of basic fibroblast growth factor-like factor by cultured human cholangiocellular carcinoma cells.

Authors:  K Matsuzaki; Y Yoshitake; M Miyagiwa; M Minemura; M Tanaka; H Sasaki; K Nishikawa
Journal:  Jpn J Cancer Res       Date:  1990-04
  2 in total

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