Development and evaluation of inocula combating high acetate concentrations during the start-up of an anaerobic digestion.

In the present study inocula to combat high acetate (CH(3)COO(-)) concentrations during start-up of an anaerobic digestion were designed and evaluated. Two strategies were followed (i) a stepwise adaptation of the engaged microorganisms within 1, 2, 4 or 6weeks, each at increasing CH(3)COO(-) concentrations of 50, 100, and finally 150mM, and (ii) shock variants, meaning a direct start with 150mM for the same durations. The stepwise adaptation for 4 and 6 weeks resulted in inocula, leading to a significant improved start-up under high CH(3)COO(-) concentrations compared to controls and shock enriched inocula. These results point to the possibility to facilitate the start-up under high CH(3)COO(-) concentrations during anaerobic digestion by addition of specific adapted inocula.