AIM: To establish an appropriate primate model of fulminant hepatic failure (FHF). METHODS: We have, for the first time, established a large animal model of FHF in Macaca mulatta by intraperitoneal infusion of amatoxin and endotoxin. Clinical features, biochemical indexes, histopathology and iconography were examined to dynamically investigate the progress and outcome of the animal model. RESULTS: Our results showed that the enzymes and serum bilirubin were markedly increased and the enzyme-bilirubin segregation emerged 36 h after toxin administration. Coagulation activity was significantly decreased. Gradually deteriorated parenchymal abnormality was detected by magnetic resonance imaging (MRI) and ultrasonography at 48 h. The liver biopsy showed marked hepatocyte steatosis and massive parenchymal necrosis at 36 h and 49 h, respectively. The autopsy showed typical yellow atrophy of the liver. Hepatic encephalopathy of the models was also confirmed by hepatic coma, MRI and pathological changes of cerebral edema. The lethal effects of the extrahepatic organ dysfunction were ruled out by their biochemical indices, imaging and histopathology. CONCLUSION: We have established an appropriate large primate model of FHF, which is closely similar to clinic cases, and can be used for investigation of the mechanism of FHF and for evaluation of potential medical therapies.
AIM: To establish an appropriate primate model of fulminant hepatic failure (FHF). METHODS: We have, for the first time, established a large animal model of FHF in Macaca mulatta by intraperitoneal infusion of amatoxin and endotoxin. Clinical features, biochemical indexes, histopathology and iconography were examined to dynamically investigate the progress and outcome of the animal model. RESULTS: Our results showed that the enzymes and serum bilirubin were markedly increased and the enzyme-bilirubin segregation emerged 36 h after toxin administration. Coagulation activity was significantly decreased. Gradually deteriorated parenchymal abnormality was detected by magnetic resonance imaging (MRI) and ultrasonography at 48 h. The liver biopsy showed marked hepatocyte steatosis and massive parenchymal necrosis at 36 h and 49 h, respectively. The autopsy showed typical yellow atrophy of the liver. Hepatic encephalopathy of the models was also confirmed by hepatic coma, MRI and pathological changes of cerebral edema. The lethal effects of the extrahepatic organ dysfunction were ruled out by their biochemical indices, imaging and histopathology. CONCLUSION: We have established an appropriate large primate model of FHF, which is closely similar to clinic cases, and can be used for investigation of the mechanism of FHF and for evaluation of potential medical therapies.
Authors: Lei Cai; Jun Weng; Lei Feng; Guolin He; Jiasheng Qin; Zhi Zhang; Yang Li; Qing Peng; Zesheng Jiang; Mingxin Pan; Yi Gao Journal: Biomed Res Int Date: 2016-12-21 Impact factor: 3.411
Authors: Lei Feng; Lei Cai; Guo-Lin He; Jun Weng; Yang Li; Ming-Xin Pan; Ze-Sheng Jiang; Qing Peng; Yi Gao Journal: World J Gastroenterol Date: 2017-11-14 Impact factor: 5.742
Authors: Yi Li; Qiong Wu; Yujia Wang; Chengxin Weng; Yuting He; Mengyu Gao; Guang Yang; Li Li; Fei Chen; Yujun Shi; Bruce P Amiot; Scott L Nyberg; Ji Bao; Hong Bu Journal: Theranostics Date: 2018-11-09 Impact factor: 11.556
Authors: Joshua Hefler; Braulio A Marfil-Garza; Rena L Pawlick; Darren H Freed; Constantine J Karvellas; David L Bigam; A M James Shapiro Journal: PeerJ Date: 2021-12-09 Impact factor: 2.984