OBJECTIVE: To assess a new immunochromatography (ICT) test that detects glutamate dehydrogenase (GDH) antigen and Clostridium difficile toxin A/B simultaneously, and to propose an algorithm for the diagnosis of C. difficile infection (CDI) based on this test. METHODS: We analysed 970 stool samples. Discrepant results between GDH and toxin A/B were resolved using toxigenic culture as the reference. RESULTS: This test enabled us to obtain a conclusive result in <30min in 93.8% of the samples. Among the discrepant results (GDH (+)/Toxin A/B (-)), 41.7% (25/60) were found to be toxigenic C. difficile by toxigenic culture. CONCLUSION: This test has a high sensitivity and specificity for the diagnosis of CDI.
OBJECTIVE: To assess a new immunochromatography (ICT) test that detects glutamate dehydrogenase (GDH) antigen and Clostridium difficile toxin A/B simultaneously, and to propose an algorithm for the diagnosis of C. difficileinfection (CDI) based on this test. METHODS: We analysed 970 stool samples. Discrepant results between GDH and toxin A/B were resolved using toxigenic culture as the reference. RESULTS: This test enabled us to obtain a conclusive result in <30min in 93.8% of the samples. Among the discrepant results (GDH (+)/Toxin A/B (-)), 41.7% (25/60) were found to be toxigenic C. difficile by toxigenic culture. CONCLUSION: This test has a high sensitivity and specificity for the diagnosis of CDI.
Authors: Analia Rodríguez Garzotto; Antonio Mérida García; Nerea Muñoz Unceta; M Mar Galera Lopez; M Ángeles Orellana-Miguel; C Vanesa Díaz-García; Susana Cortijo-Cascajares; Hernán Cortes-Funes; M Teresa Agulló-Ortuño Journal: Support Care Cancer Date: 2014-11-20 Impact factor: 3.603
Authors: Julia Origüen; Mario Fernández-Ruiz; Carlos Lumbreras; María Ángeles Orellana; Francisco López-Medrano; Tamara Ruiz-Merlo; Rafael San Juan; Ana García-Reyne; Esther González; Natalia Polanco; Estela Paz-Artal; Amado Andrés; José María Aguado Journal: Infection Date: 2015-02-13 Impact factor: 3.553
Authors: Lee F Schroeder; Elizabeth Robilotti; Lance R Peterson; Niaz Banaei; David W Dowdy Journal: J Clin Microbiol Date: 2013-11-27 Impact factor: 5.948
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