Literature DB >> 22336744

Fructanase and fructosyltransferase activity of non-Saccharomyces yeasts isolated from fermenting musts of Mezcal.

Javier Arrizon1, Sandrine Morel, Anne Gschaedler, Pierre Monsan.   

Abstract

Fructanase and fructosyltransferase are interesting for the tequila process and prebiotics production (functional food industry). In this study, one hundred thirty non-Saccharomyces yeasts isolated from "Mezcal de Oaxaca" were screened for fructanase and fructosyltransferase activity. On solid medium, fifty isolates grew on Agave tequilana fructans (ATF), inulin or levan. In liquid media, inulin and ATF induced fructanase activities of between 0.02 and 0.27U/ml depending of yeast isolate. High fructanase activity on sucrose was observed for Kluyveromyces marxianus and Torulaspora delbrueckii, while the highest fructanase activity on inulin and ATF was observed for Issatchenkia orientalis, Cryptococcus albidus, and Candida apicola. Zygosaccharomyces bisporus and Candida boidinii had a high hydrolytic activity on levan. Sixteen yeasts belonging to K. marxianus, T. delbrueckii and C. apicola species were positive for fructosyltransferase activity. Mezcal microbiota proved to showed to be a source for new fructanase and fructosyltransferases with potential application in the tequila and food industry.
Copyright © 2012 Elsevier Ltd. All rights reserved.

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Year:  2012        PMID: 22336744     DOI: 10.1016/j.biortech.2012.01.112

Source DB:  PubMed          Journal:  Bioresour Technol        ISSN: 0960-8524            Impact factor:   9.642


  10 in total

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5.  Genome Sequence of Torulaspora delbrueckii NRRL Y-50541, Isolated from Mezcal Fermentation.

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Authors:  Raúl A Ortiz-Merino; Javier A Varela; Aisling Y Coughlan; Hisashi Hoshida; Wendel B da Silveira; Caroline Wilde; Niels G A Kuijpers; Jan-Maarten Geertman; Kenneth H Wolfe; John P Morrissey
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  10 in total

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