Literature DB >> 22327205

A single centrifugation method for isolating fat droplets from cells and tissues.

Lydia-Ann L S Harris1, Trevor M Shew1, James R Skinner1, Nathan E Wolins2.   

Abstract

Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation step in a standard microcentrifuge. This procedure reproducibly yields three fractions highly enriched in either FDs, soluble cellular components, or sedimentable organelles/membranes.

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Year:  2012        PMID： 22327205      PMCID： PMC3329379          DOI： 10.1194/jlr.D023598

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  9 in total

1.  S3-12, Adipophilin, and TIP47 package lipid in adipocytes.

Authors:  Nathan E Wolins; Benjamin K Quaynor; James R Skinner; Marissa J Schoenfish; Anatoly Tzekov; Perry E Bickel
Journal:  J Biol Chem       Date:  2005-02-24       Impact factor: 5.157

2.  OXPAT/PAT-1 is a PPAR-induced lipid droplet protein that promotes fatty acid utilization.

Authors:  Nathan E Wolins; Benjamin K Quaynor; James R Skinner; Anatoly Tzekov; Michelle A Croce; Matthew C Gropler; Vijayalakshmi Varma; Aiwei Yao-Borengasser; Neda Rasouli; Philip A Kern; Brian N Finck; Perry E Bickel
Journal:  Diabetes       Date:  2006-12       Impact factor: 9.461

3.  Perilipin A increases triacylglycerol storage by decreasing the rate of triacylglycerol hydrolysis.

Authors:  D L Brasaemle; B Rubin; I A Harten; J Gruia-Gray; A R Kimmel; C Londos
Journal:  J Biol Chem       Date:  2000-12-08       Impact factor: 5.157

4.  TIP47 associates with lipid droplets.

Authors:  N E Wolins; B Rubin; D L Brasaemle
Journal:  J Biol Chem       Date:  2000-11-17       Impact factor: 5.157

5.  Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3T3-L1 adipocytes.

Authors:  Dawn L Brasaemle; Georgia Dolios; Lawrence Shapiro; Rong Wang
Journal:  J Biol Chem       Date:  2004-08-27       Impact factor: 5.157

6.  Adipocyte protein S3-12 coats nascent lipid droplets.

Authors:  Nathan E Wolins; James R Skinner; Marissa J Schoenfish; Anatoly Tzekov; Kenneth G Bensch; Perry E Bickel
Journal:  J Biol Chem       Date:  2003-07-02       Impact factor: 5.157

7.  Perilipin A mediates the reversible binding of CGI-58 to lipid droplets in 3T3-L1 adipocytes.

Authors:  Vidya Subramanian; Alexis Rothenberg; Carlos Gomez; Alex W Cohen; Anne Garcia; Sucharita Bhattacharyya; Lawrence Shapiro; Georgia Dolios; Rong Wang; Michael P Lisanti; Dawn L Brasaemle
Journal:  J Biol Chem       Date:  2004-08-02       Impact factor: 5.157

8.  Diacylglycerol enrichment of endoplasmic reticulum or lipid droplets recruits perilipin 3/TIP47 during lipid storage and mobilization.

Authors:  James R Skinner; Trevor M Shew; Danielle M Schwartz; Anatoly Tzekov; Christin M Lepus; Nada A Abumrad; Nathan E Wolins
Journal:  J Biol Chem       Date:  2009-09-11       Impact factor: 5.157

9.  Dynamic and differential regulation of proteins that coat lipid droplets in fatty liver dystrophic mice.

Authors:  Angela M Hall; Elizabeth M Brunt; Zhouji Chen; Navin Viswakarma; Janardan K Reddy; Nathan E Wolins; Brian N Finck
Journal:  J Lipid Res       Date:  2009-09-11       Impact factor: 5.922
  9 in total
  12 in total

1.  Dgat1 and Dgat2 regulate enterocyte triacylglycerol distribution and alter proteins associated with cytoplasmic lipid droplets in response to dietary fat.

Authors:  Yu-Han Hung; Alicia L Carreiro; Kimberly K Buhman
Journal:  Biochim Biophys Acta Mol Cell Biol Lipids       Date:  2017-02-27       Impact factor: 4.698

2.  Identification of Lipid Droplet Proteomes by Proximity Labeling Proteomics Using APEX2.

Authors:  Kirill Bersuker; James A Olzmann
Journal:  Methods Mol Biol       Date:  2019

Review 3.  Biology and pathobiology of lipid droplets and their potential role in the protection of the organ of Corti.

Authors:  Raul A Urrutia; Federico Kalinec
Journal:  Hear Res       Date:  2015-05-15       Impact factor: 3.208

4.  The lipid droplet-associated protein perilipin 3 facilitates hepatitis C virus-driven hepatic steatosis.

Authors:  Daniel Ferguson; Jun Zhang; Matthew A Davis; Robert N Helsley; Lise-Lotte Vedin; Richard G Lee; Rosanne M Crooke; Mark J Graham; Daniela S Allende; Paolo Parini; J Mark Brown
Journal:  J Lipid Res       Date:  2016-12-10       Impact factor: 5.922

5.  Mesoderm-specific transcript localization in the ER and ER-lipid droplet interface supports a role in adipocyte hypertrophy.

Authors:  Igor Prudovsky; Rea P Anunciado-Koza; Chester G Jacobs; Doreen Kacer; Matthew E Siviski; Robert A Koza
Journal:  J Cell Biochem       Date:  2017-12-04       Impact factor: 4.429

6.  Aifm2, a NADH Oxidase, Supports Robust Glycolysis and Is Required for Cold- and Diet-Induced Thermogenesis.

Authors:  Hai P Nguyen; Danielle Yi; Frances Lin; Jose A Viscarra; Chihiro Tabuchi; Katina Ngo; Gawon Shin; Angus Yiu-Fai Lee; Yuhui Wang; Hei Sook Sul
Journal:  Mol Cell       Date:  2020-01-14       Impact factor: 17.970

7.  Perilipin 1 moves between the fat droplet and the endoplasmic reticulum.

Authors:  James R Skinner; Lydia-Ann L S Harris; Trevor M Shew; Nada A Abumrad; Nathan E Wolins
Journal:  Adipocyte       Date:  2013-04-01       Impact factor: 4.534

8.  Fatty Acid and Lipopolysaccharide Effect on Beta Cells Proteostasis and its Impact on Insulin Secretion.

Authors:  Paloma Acosta-Montaño; Eustolia Rodríguez-Velázquez; Esmeralda Ibarra-López; Héctor Frayde-Gómez; Jaime Mas-Oliva; Blanca Delgado-Coello; Ignacio A Rivero; Manuel Alatorre-Meda; Jorge Aguilera; Lizbeth Guevara-Olaya; Victor García-González
Journal:  Cells       Date:  2019-08-13       Impact factor: 6.600

9.  Proteomic Characterization of Cytoplasmic Lipid Droplets in Human Metastatic Breast Cancer Cells.

Authors:  Alyssa S Zembroski; Chaylen Andolino; Kimberly K Buhman; Dorothy Teegarden
Journal:  Front Oncol       Date:  2021-06-01       Impact factor: 6.244

10.  Abrogating monoacylglycerol acyltransferase activity in liver improves glucose tolerance and hepatic insulin signaling in obese mice.

Authors:  Angela M Hall; Nisreen Soufi; Kari T Chambers; Zhouji Chen; George G Schweitzer; Kyle S McCommis; Derek M Erion; Mark J Graham; Xiong Su; Brian N Finck
Journal:  Diabetes       Date:  2014-03-04       Impact factor: 9.461
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