OBJECTIVES: Validation of the Architect 25-OH vitamin D assay. DESIGN AND METHODS: Determination of repeatability, reproducibility, accuracy profile and 25(OH)-vitamin D2 recovery on native samples. Comparison with DiaSorin Liaison and RIA. RESULTS AND CONCLUSION: Coefficients of variation: <6% (13.6 ng/mL) and 2.2% (78.1 ng/mL). Functional sensitivity: 5 ng/mL. Accuracy profile shows that the method is validated between 13.6 and 78.1 ng/mL. Recovery of 25(OH)D2: 75,8%( 95% CI: 61.9-89.7%). Good correlation with DiaSorin RIA and Liaison <50 ng/mL; above this threshold a systematic positive bias was observed.
OBJECTIVES: Validation of the Architect 25-OH vitamin D assay. DESIGN AND METHODS: Determination of repeatability, reproducibility, accuracy profile and 25(OH)-vitamin D2 recovery on native samples. Comparison with DiaSorin Liaison and RIA. RESULTS AND CONCLUSION: Coefficients of variation: <6% (13.6 ng/mL) and 2.2% (78.1 ng/mL). Functional sensitivity: 5 ng/mL. Accuracy profile shows that the method is validated between 13.6 and 78.1 ng/mL. Recovery of 25(OH)D2: 75,8%( 95% CI: 61.9-89.7%). Good correlation with DiaSorin RIA and Liaison <50 ng/mL; above this threshold a systematic positive bias was observed.
Authors: Melissa S Putman; Joshua F Baker; Ahmet Uluer; Karen Herlyn; Allen Lapey; Leonard Sicilian; Angela Pizzo Tillotson; Catherine M Gordon; Peter A Merkel; Joel S Finkelstein Journal: J Cyst Fibros Date: 2015-02-16 Impact factor: 5.482
Authors: Jennifer Jao; Laura Freimanis; Marisa M Mussi-Pinhata; Rachel A Cohen; Jacqueline P Monteiro; Maria L Cruz; Rhoda S Sperling; Andrea Branch; George K Siberry Journal: Int J Gynaecol Obstet Date: 2015-04-11 Impact factor: 3.561