| Literature DB >> 2230142 |
N Ida1, S Sakurai, T Hosaka, K Hosoi, T Kunitomo, Y Matsuura, M Kohase.
Abstract
An enzyme-linked immunosorbent assay has been developed to measure human interleukin-6. The assay, based on the avidin-biotin amplified two-step sandwich method, is quick (requiring 4.5 h), sensitive (detecting 9.5 pg/ml) and satisfactory in reproducibility and specificity. It shows good correspondence with the results of bioassays, and it is not affected by serum and plasma components. These results indicate that this ELISA is suitable for application to clinical samples, which is a major advantage over the widely used bioassays.Entities:
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Year: 1990 PMID: 2230142 DOI: 10.1016/0022-1759(90)90369-7
Source DB: PubMed Journal: J Immunol Methods ISSN: 0022-1759 Impact factor: 2.303