Literature DB >> 22294105

Functional study of immature dendritic cells co-transfected with IL-10 and TGF-beta 1 genes in vitro.

Lihong Chen1, Minglian Qiu, Wubing He, Aimin Huang, Jingfeng Liu.   

Abstract

Dendritic cells (DC) have important functions in T cell immunity and T cell tolerance. Previous studies suggest that immature dendritic cells (imDCs) might be involved in the induction of peripheral T cell tolerance. While interleukin-10 (IL-10) functions at different levels of the immune response, transforming growth factor-beta 1 (TGF-beta 1) is considered to be a key factor in immune tolerance. In this study, we investigated the effects of immature DC (imDC) co-transfected with IL-10 and TGF-beta 1 genes (IL-10-TGF-beta 1-imDC) on inducing immune tolerance. Moreover, we compared the effects of IL-10-TGF-beta 1-imDC with IL-10 transfected imDC (IL-10-imDC) and TGF-beta 1-transfected imDC (TGF-beta 1-imDC), respectively. IL-10-TGF-beta 1-imDC resulted in the down-regulation of MHC class II, CD80 and CD86. IL-10-TGF-beta 1-imDC could induce T cell hyporesponsiveness, and was reluctant to proliferate. IL-10-TGF-beta 1-imDC was more effective than IL-10-imDC and TGF-beta 1-imDC, respectively. In summary, co-expression of IL-10 and TGF-beta 1 affected the immunity of imDCs and enhanced their tolerogenicity. It might be a promising therapy for donor-specific tolerance after organ transplantation.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 22294105     DOI： 10.1007/s11033-012-1468-4

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  26 in total

Review 1.  TGF-beta and immune cells: an important regulatory axis in the tumor microenvironment and progression.

Authors:  Li Yang; Yanli Pang; Harold L Moses
Journal:  Trends Immunol       Date:  2010-06-01       Impact factor: 16.687

2.  TGF-beta is required to maintain the pool of immature Langerhans cells in the epidermis.

Authors:  Junda M Kel; Mathilde J H Girard-Madoux; Boris Reizis; Björn E Clausen
Journal:  J Immunol       Date:  2010-08-16       Impact factor: 5.422

3.  Toll-like receptor 4-dependent activation of dendritic cells by beta-defensin 2.

Authors:  Arya Biragyn; Pier Adelchi Ruffini; Cynthia A Leifer; Elena Klyushnenkova; Alexander Shakhov; Oleg Chertov; Aiko K Shirakawa; Joshua M Farber; David M Segal; Joost J Oppenheim; Larry W Kwak
Journal:  Science       Date:  2002-11-01       Impact factor: 47.728

4.  TGF-beta1 gene-modified, immature dendritic cells delay the development of inflammatory bowel disease by inducing CD4(+)Foxp3(+) regulatory T cells.

Authors:  Zhijian Cai; Wei Zhang; Min Li; Yinpu Yue; Fei Yang; Lei Yu; Xuetao Cao; Jianli Wang
Journal:  Cell Mol Immunol       Date:  2010-01       Impact factor: 11.530

5.  Up-regulation of IL-10 expression in dendritic cells is involved in Trichosanthin-induced immunosuppression.

Authors:  Xiaorong Zhou; Neng Yang; Liming Lu; Qing Ding; Zhijun Jiao; Yun Zhou; Kuang-Yen Chou
Journal:  Immunol Lett       Date:  2007-04-19       Impact factor: 3.685

6.  Distinct contributions of TNF and LT cytokines to the development of dendritic cells in vitro and their recruitment in vivo.

Authors:  Koichiro Abe; Felix O Yarovinsky; Takaya Murakami; Alexander N Shakhov; Alexei V Tumanov; Daisuke Ito; Ludmila N Drutskaya; Klaus Pfeffer; Dmitry V Kuprash; Kristin L Komschlies; Sergei A Nedospasov
Journal:  Blood       Date:  2003-02-15       Impact factor: 22.113

7.  Identification of IL-10 and TGF-beta transcripts involved in the inhibition of T-lymphocyte proliferation during cell contact with human mesenchymal stem cells.

Authors:  Aisha Nasef; Alain Chapel; Christelle Mazurier; Sandrine Bouchet; Manuel Lopez; Noelle Mathieu; Luc Sensebé; Yizhuo Zhang; Norbert-Claude Gorin; Dominique Thierry; Loïc Fouillard
Journal:  Gene Expr       Date:  2007

8.  TGF-beta induces apoptosis in human B cells by transcriptional regulation of BIK and BCL-XL.

Authors:  L C Spender; D I O'Brien; D Simpson; D Dutt; C D Gregory; M J Allday; L J Clark; G J Inman
Journal:  Cell Death Differ       Date:  2009-01-09       Impact factor: 15.828

Review 9.  Dendritic cells as immune regulators: the mouse model.

Authors:  K L Griffiths; H C O'Neill
Journal:  J Cell Mol Med       Date:  2008-06-09       Impact factor: 5.310

10.  Immunosuppression induced by immature dendritic cells is mediated by TGF-beta/IL-10 double-positive CD4+ regulatory T cells.

Authors:  N Cools; V F I Van Tendeloo; E L J M Smits; M Lenjou; G Nijs; D R Van Bockstaele; Z N Berneman; P Ponsaerts
Journal:  J Cell Mol Med       Date:  2008-04       Impact factor: 5.310
View more
  5 in total

1.  The siRNA cocktail targeting interleukin 10 receptor and transforming growth factor-β receptor on dendritic cells potentiates tumour antigen-specific CD8(+) T cell immunity.

Authors:  Y-H Ahn; S-O Hong; J H Kim; K H Noh; K-H Song; Y-H Lee; J-H Jeon; D-W Kim; J H Seo; T W Kim
Journal:  Clin Exp Immunol       Date:  2015-05-17       Impact factor: 4.330

2.  Immune protective effect of human alpha-1-antitrypsin gene during β cell transplantation in diabetic mice.

Authors:  Lu Yang; Yu-Ting Liao; Xiao-Fei Yang; Li-Wei Reng; Hui Qi; Fu-Rong Li
Journal:  Immunol Res       Date:  2015-05       Impact factor: 2.829

Review 3.  Regulatory Dendritic Cells, T Cell Tolerance, and Dendritic Cell Therapy for Immunologic Disease.

Authors:  Sara Ness; Shiming Lin; John R Gordon
Journal:  Front Immunol       Date:  2021-03-10       Impact factor: 7.561

Review 4.  Regulatory dendritic cells for immunotherapy in immunologic diseases.

Authors:  John R Gordon; Yanna Ma; Laura Churchman; Sara A Gordon; Wojciech Dawicki
Journal:  Front Immunol       Date:  2014-01-31       Impact factor: 7.561

Review 5.  Regulation of Natural Killer Cell Function by STAT3.

Authors:  Nicholas A Cacalano
Journal:  Front Immunol       Date:  2016-04-11       Impact factor: 7.561
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.