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Literature DB >> 2229389

DNA probes for the identification of Nocardia asteroides.

Abstract

DNA probes for the rapid identification of Nocardia asteroides were obtained by constructing a genomic library of strain GUH-2 in the lambda cloning vector EMBL3. Of 50 recombinant clones tested, 2 were identified that hybridized with 31% of the N. asteroides strains in a reference collection without cross-hybridization with related members of the Actinomycetales. Additional libraries were then generated from selected strains of N. asteroides that had failed to hybridize with any of the GUH-2 clones. Four additional clones were obtained from these strains which, when pooled, provided DNA probes specific for all of the N. asteroides strains tested.

Entities: Species

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Substances：
DNA Probes

Year: 1990 PMID： 2229389 PMCID： PMC268107 DOI： 10.1128/jcm.28.9.2082-2086.1990

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

25 in total

1. Selective enrichment of specific DNA, cDNA and RNA sequences using biotinylated probes, avidin and copper-chelate agarose.

Authors: A A Welcher; A R Torres; D C Ward
Journal: Nucleic Acids Res Date: 1986-12-22 Impact factor: 16.971

DNA probes for the identification of Nocardia asteroides.

1. Selective enrichment of specific DNA, cDNA and RNA sequences using biotinylated probes, avidin and copper-chelate agarose.

2. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.

3. Rapid diagnosis of nocardiosis with an enzyme immunoassay.

4. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.

5. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

6. New bacteriophage lambda vectors with positive selection for cloned inserts.

7. Lambda replacement vectors carrying polylinker sequences.

8. Nocardial infections in the United States, 1972-1974.

9. Immunodiffusion studies of some Nocardia strains.

10. Numerical analysis of the taxonomy of Nocardiae and Rhodococci.

1. A rapid and gentle method for isolation of genomic DNA from pathogenic Nocardia spp.

2. Fatty acid characterization of rapidly growing pathogenic aerobic actinomycetes as a means of identification.

Review 3. Clinical and laboratory features of the Nocardia spp. based on current molecular taxonomy.

Review 4. The medically important aerobic actinomycetes: epidemiology and microbiology.