OBJECTIVES: To compare the plasma concentrations of matrix metalloproteinase (MMP)-9, tissue inhibitor of MMP (TIMP)-1, MMP-8, and myeloperoxidase (MPO) for obese and lean women. DESIGN AND METHODS: We recruited 30 lean and 36 obese women without comorbidities. The MMP-9, TIMP-1, and MMP-8 levels were measured using enzyme-linked immunosorbent assay (ELISA). MPO activity was assessed by a colorimetric assay. RESULTS: Obese women had higher MMP-9 levels and MMP-9:TIMP-1 ratios than lean women. Conversely, the MMP-8 levels and MMP-8:TIMP-1 ratios in the obese women were significantly lower than those in the lean women despite neutrophil activation, which was assessed by MPO activity. CONCLUSION: We observed that MMP-9 and MMP-8 had distinct profiles, which suggested that these 2 enzymes play different roles in obesity.
OBJECTIVES: To compare the plasma concentrations of matrix metalloproteinase (MMP)-9, tissue inhibitor of MMP (TIMP)-1, MMP-8, and myeloperoxidase (MPO) for obese and lean women. DESIGN AND METHODS: We recruited 30 lean and 36 obesewomen without comorbidities. The MMP-9, TIMP-1, and MMP-8 levels were measured using enzyme-linked immunosorbent assay (ELISA). MPO activity was assessed by a colorimetric assay. RESULTS:Obesewomen had higher MMP-9 levels and MMP-9:TIMP-1 ratios than lean women. Conversely, the MMP-8 levels and MMP-8:TIMP-1 ratios in the obesewomen were significantly lower than those in the lean women despite neutrophil activation, which was assessed by MPO activity. CONCLUSION: We observed that MMP-9 and MMP-8 had distinct profiles, which suggested that these 2 enzymes play different roles in obesity.
Authors: Wojciech Szczęsny; Magdalena Kuligowska-Prusińska; Stanisław Dąbrowiecki; Jakub Szmytkowski; Adrian Reśliński; Maciej Słupski Journal: J Zhejiang Univ Sci B Date: 2018 Jan. Impact factor: 3.066
Authors: Mohammad Ali; Shahana Jasmin; Mohammad Fariduddin; Sheikh M K Alam; M I Arslan; Subrata K Biswas Journal: Mol Biol Rep Date: 2018-07-28 Impact factor: 2.316
Authors: Marcelo R Luizon; Vanessa A Belo; Karla S Fernandes; Vanessa L Andrade; Jose E Tanus-Santos; Valeria C Sandrim Journal: Mol Biol Rep Date: 2016-05-05 Impact factor: 2.316
Authors: Josune Olza; Concepcion M Aguilera; Mercedes Gil-Campos; Rosaura Leis; Gloria Bueno; Maria D Martínez-Jiménez; Miguel Valle; Ramon Cañete; Rafael Tojo; Luis A Moreno; Angel Gil Journal: Diabetes Care Date: 2012-08-21 Impact factor: 19.112