| Literature DB >> 22275140 |
Daniel Cuthbertson1, Jasenka Piljac-Žegarac, Bernd Markus Lange.
Abstract
Herein we report on an improved method for the microscale extraction of huperzine A (HupA), an acetylcholinesterase-inhibiting alkaloid, from as little as 3 mg of tissue homogenate from the clubmoss Huperzia squarrosa (G. Forst.) Trevis with 99.95% recovery. We also validated a novel UHPLC-QTOF-MS method for the high-throughput analysis of H. squarrosa extracts in only 6 min, which, in combination with the very low limit of detection (20 pg on column) and the wide linear range for quantification (20-10,000 pg on column), allow for a highly efficient screening of extracts containing varying amounts of HupA. Utilization of this methodology has the potential to conserve valuable plant resources.Entities:
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Year: 2012 PMID： 22275140 PMCID： PMC3337887 DOI： 10.1002/bmc.2677
Source DB: PubMed Journal: Biomed Chromatogr ISSN： 0269-3879 Impact factor: 1.902