| Literature DB >> 22264005 |
Khalilah G Reddie1, William H Humphries, Charlo P Bain, Christine K Payne, Melissa L Kemp, Niren Murthy.
Abstract
In this report we present a new chemical probe, 3-HTC, that can reversibly and ratiometrically measure the thiol-disulfide equilibrium of biological systems. 3-HTC is composed of a coumarin that has a thiolate directly conjugated to its extended aromatic π system while formation of a disulfide attenuates this conjugation. The fluorescence and absorption properties of 3-HTC are therefore very sensitive to the redox state of its thiol. 3-HTC reacts reversibly with thiols and disulfides enabling its use to measure dynamic GSH/GSSH ratios in vitro as well as to monitor the reversible redox status of whole cell lysates.Entities:
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Year: 2012 PMID： 22264005 PMCID： PMC3291176 DOI： 10.1021/ol203105c
Source DB: PubMed Journal: Org Lett ISSN： 1523-7052 Impact factor: 6.005