| Literature DB >> 22237525 |
Paula F Campos1, Thomas M P Gilbert.
Abstract
The principal challenges facing PCR-based analyses of DNA extracted from formalin-fixed materials are fragmentation of the DNA and cross-linked protein-DNA complexes. Here, we present an efficient protocol to extract DNA from formalin-fixed or paraffin-embedded tissues (FFPE). In this protocol, protein-DNA cross-links are reversed using heat and alkali treatment, yielding significantly longer fragments and larger amounts of PCR-amplifiable DNA than standard DNA extraction protocols.Entities:
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Year: 2012 PMID： 22237525 DOI： 10.1007/978-1-61779-516-9_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745