AIM: To investigate the growth effects of 4-phenyl butyric acid (PBA) on human gastric carcinoma cells and their mechanisms. METHODS: Moderately-differentiated human gastric carcinoma SGC-7901 and lowly-differentiated MGC-803 cells were treated with 5, 10, 20, 40, and 60 μmol/L PBA for 1-4 d. Cell proliferation was detected using the MTT colorimetric assay. Cell cycle distributions were examined using flow cytometry. RESULTS: The proliferation of gastric carcinoma cells was inhibited by PBA in a dose- and time-dependent fashion. Flow cytometry showed that SGC-7901 cells treated with low concentrations of PBA were arrested at the G₀/G₁ phase, whereas cells treated with high concentrations of PBA were arrested at the G₂/M phase. Although MGC-803 cells treated with low concentrations of PBA were also arrested at the G₀/ G₁ phase, cells treated with high concentrations of PBA were arrested at the S phase. CONCLUSION: The growth inhibitory effect of PBA on gastric cancer cells is associated with alteration of the cell cycle. For moderately-differentiated gastric cancer cells, the cell cycle was arrested at the G₀ /G₁ and G₂/M phases. For lowly-differentiated gastric cancer cells, the cell cycle was arrested at the G₀/G₁ and S phases.
AIM: To investigate the growth effects of 4-phenyl butyric acid (PBA) on humangastric carcinoma cells and their mechanisms. METHODS: Moderately-differentiated humangastric carcinoma SGC-7901 and lowly-differentiated MGC-803 cells were treated with 5, 10, 20, 40, and 60 μmol/L PBA for 1-4 d. Cell proliferation was detected using the MTT colorimetric assay. Cell cycle distributions were examined using flow cytometry. RESULTS: The proliferation of gastric carcinoma cells was inhibited by PBA in a dose- and time-dependent fashion. Flow cytometry showed that SGC-7901 cells treated with low concentrations of PBA were arrested at the G₀/G₁ phase, whereas cells treated with high concentrations of PBA were arrested at the G₂/M phase. Although MGC-803 cells treated with low concentrations of PBA were also arrested at the G₀/ G₁ phase, cells treated with high concentrations of PBA were arrested at the S phase. CONCLUSION: The growth inhibitory effect of PBA on gastric cancer cells is associated with alteration of the cell cycle. For moderately-differentiated gastric cancer cells, the cell cycle was arrested at the G₀ /G₁ and G₂/M phases. For lowly-differentiated gastric cancer cells, the cell cycle was arrested at the G₀/G₁ and S phases.
Authors: Irina Svechnikova; Steven G Gray; Jurgita Kundrotiene; Frida Ponthan; Per Kogner; Tomas J Ekström Journal: Int J Oncol Date: 2003-03 Impact factor: 5.650
Authors: A Satoh; M Toyota; F Itoh; T Kikuchi; T Obata; Y Sasaki; H Suzuki; A Yawata; M Kusano; M Fujita; M Hosokawa; K Yanagihara; T Tokino; K Imai Journal: Br J Cancer Date: 2002-06-05 Impact factor: 7.640
Authors: R Kuefer; M D Hofer; V Altug; C Zorn; F Genze; K Kunzi-Rapp; R E Hautmann; J E Gschwend Journal: Br J Cancer Date: 2004-01-26 Impact factor: 7.640
Authors: Ritesh K Srivastava; Changzhao Li; Sandeep C Chaudhary; Mary E Ballestas; Craig A Elmets; David J Robbins; Sadis Matalon; Jessy S Deshane; Farrukh Afaq; David R Bickers; Mohammad Athar Journal: Toxicol Appl Pharmacol Date: 2013-08-14 Impact factor: 4.219