| Literature DB >> 22227115 |
Jing Zhang1, Christophe Rouillon, Melina Kerou, Judith Reeks, Kim Brugger, Shirley Graham, Julia Reimann, Giuseppe Cannone, Huanting Liu, Sonja-Verena Albers, James H Naismith, Laura Spagnolo, Malcolm F White.
Abstract
The prokaryotic clusters of regularly interspaced palindromic repeats (CRISPR) system utilizes genomically encoded CRISPR RNA (crRNA), derived from invading viruses and incorporated into ribonucleoprotein complexes with CRISPR-associated (CAS) proteins, to target and degrade viral DNA or RNA on subsequent infection. RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse "payload" of targeting crRNA. The crystal structure of Cmr7 and low-resolution structure of the complex are presented. S. solfataricus CMR cleaves RNA targets in an endonucleolytic reaction at UA dinucleotides. This activity is dependent on the 8 nt repeat-derived 5' sequence in the crRNA, but not on the presence of a protospacer-associated motif (PAM) in the target. Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets.Entities:
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Year: 2012 PMID： 22227115 PMCID： PMC3381847 DOI： 10.1016/j.molcel.2011.12.013
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970