BACKGROUND: Searching for additional therapeutic tools to fight breast cancer, we investigated the effects of vitamin D-binding protein-derived macrophage activating factor (DBP-MAF, also known as GcMAF) on a human breast cancer cell line (MCF-7). MATERIALS AND METHODS: The effects of DBP-MAF on proliferation, morphology, vimentin expression and angiogenesis were studied by cell proliferation assay, phase-contrast microscopy, immunohistochemistry and western blotting, and chorioallantoic membrane (CAM) assay. RESULTS: DBP-MAF inhibited human breast cancer cell proliferation and cancer cell-stimulated angiogenesis. MCF-7 cells treated with DBP-MAF predominantly grew in monolayer and appeared to be well adherent to each other and to the well surface. Exposure to DBP-MAF significantly reduced vimentin expression, indicating a reversal of the epithelial/mesenchymal transition, a hallmark of human breast cancer progression. CONCLUSION: These results are consistent with the hypothesis that the known anticancer efficacy of DBP-MAF can be ascribed to different biological properties of the molecule that include inhibition of tumour-induced angiogenesis and direct inhibition of cancer cell proliferation, migration and metastatic potential.
BACKGROUND: Searching for additional therapeutic tools to fight breast cancer, we investigated the effects of vitamin D-binding protein-derived macrophage activating factor (DBP-MAF, also known as GcMAF) on a humanbreast cancer cell line (MCF-7). MATERIALS AND METHODS: The effects of DBP-MAF on proliferation, morphology, vimentin expression and angiogenesis were studied by cell proliferation assay, phase-contrast microscopy, immunohistochemistry and western blotting, and chorioallantoic membrane (CAM) assay. RESULTS:DBP-MAF inhibited humanbreast cancer cell proliferation and cancer cell-stimulated angiogenesis. MCF-7 cells treated with DBP-MAF predominantly grew in monolayer and appeared to be well adherent to each other and to the well surface. Exposure to DBP-MAF significantly reduced vimentin expression, indicating a reversal of the epithelial/mesenchymal transition, a hallmark of humanbreast cancer progression. CONCLUSION: These results are consistent with the hypothesis that the known anticancer efficacy of DBP-MAF can be ascribed to different biological properties of the molecule that include inhibition of tumour-induced angiogenesis and direct inhibition of cancer cell proliferation, migration and metastatic potential.
Authors: Chen Yuan; Irene M Shui; Kathryn M Wilson; Meir J Stampfer; Lorelei A Mucci; Edward L Giovannucci Journal: Int J Cancer Date: 2018-12-06 Impact factor: 7.396
Authors: Stephanie J Weinstein; Alison M Mondul; William Kopp; Helen Rager; Jarmo Virtamo; Demetrius Albanes Journal: Int J Cancer Date: 2012-12-27 Impact factor: 7.396
Authors: Chen Yuan; Mingyang Song; Yin Zhang; Brian M Wolpin; Jeffrey A Meyerhardt; Shuji Ogino; Bruce W Hollis; Andrew T Chan; Charles S Fuchs; Kana Wu; Molin Wang; Stephanie A Smith-Warner; Edward L Giovannucci; Kimmie Ng Journal: Cancer Epidemiol Biomarkers Prev Date: 2020-09-11 Impact factor: 4.254
Authors: Suneil Malik; Lei Fu; David James Juras; Mohamed Karmali; Betty Y L Wong; Agnes Gozdzik; David E C Cole Journal: Crit Rev Clin Lab Sci Date: 2013-02-22 Impact factor: 6.250