| Literature DB >> 22207201 |
Sylvain D Ethier1, Hisashi Miura, Josée Dostie.
Abstract
It has been known for some time that eukaryotic genomic DNA is packaged in the form of highly organized chromatin in vivo. This organization is important not only to reduce the length of chromosomes during interphase but also because it represents a type of higher-order genome regulation mechanism. Indeed, spatial chromatin architecture is known to be important for transcription, DNA replication and repair. Chromosome structure can be observed at different scales and studied with a variety of complementary techniques. For example, microscopy can provide single cell information while technologies such as the chromosome conformation capture (3C) method and its derivatives can yield higher-resolution data from cell populations. In this review, we report on the biological questions addressed with 3C and 3C-related techniques and what has been uncovered to date. We also explore what these methods may further reveal about the regulation of genomic DNA activities.Mesh：
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Year: 2011 PMID： 22207201 DOI： 10.1016/j.bbagrm.2011.12.004
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002