BACKGROUND AND AIMS: A number of theories have been put forward to clarify the etiology of colorectal cancer (CRC), such as genetic alterations and cytokine production. A combination of inflammatory cytokines has an important role in cancer development. The aim of our study was to screen for alterations located in promoter and exons of IL-16 gene sequence, to determine the distribution of genotypes in individuals with CRC and healthy controls in a sample of Iranian population. METHODS: The case group consisted of 260 individuals with colorectal cancer and the control group included 405 healthy individuals. Three IL-16 gene polymorphisms (rs4072111, rs11556218, rs4778889) were genotyped using PCR-RFLP method. RFLP results were confirmed by direct sequencing. RESULTS: A significant association between rs11556218 SNP in the IL-16 gene and the risk of CRC was found. The TG genotype of rs11556218 T/G polymorphism showed significant association with a 1.75 fold increased risk of CRC (P=0.005; adjusted OR: 1.759; 95% CI: 1.191-2.598). In addition a significant association between CC genotype of rs4778889 T/C polymorphism and decreased risk of CRC in male subjects (P=0.045; adjusted OR: 0.192; 95% CI: 0.038-0.967) was determined. CONCLUSION: This study is the first report of IL-16 gene polymorphisms among CRC patients from Iran. Our results suggest an influence of rs11556218 T > G and rs4778889 T/C polymorphisms on the altered risk of CRC.
BACKGROUND AND AIMS: A number of theories have been put forward to clarify the etiology of colorectal cancer (CRC), such as genetic alterations and cytokine production. A combination of inflammatory cytokines has an important role in cancer development. The aim of our study was to screen for alterations located in promoter and exons of IL-16 gene sequence, to determine the distribution of genotypes in individuals with CRC and healthy controls in a sample of Iranian population. METHODS: The case group consisted of 260 individuals with colorectal cancer and the control group included 405 healthy individuals. Three IL-16 gene polymorphisms (rs4072111, rs11556218, rs4778889) were genotyped using PCR-RFLP method. RFLP results were confirmed by direct sequencing. RESULTS: A significant association between rs11556218 SNP in the IL-16 gene and the risk of CRC was found. The TG genotype of rs11556218 T/G polymorphism showed significant association with a 1.75 fold increased risk of CRC (P=0.005; adjusted OR: 1.759; 95% CI: 1.191-2.598). In addition a significant association between CC genotype of rs4778889 T/C polymorphism and decreased risk of CRC in male subjects (P=0.045; adjusted OR: 0.192; 95% CI: 0.038-0.967) was determined. CONCLUSION: This study is the first report of IL-16 gene polymorphisms among CRC patients from Iran. Our results suggest an influence of rs11556218 T > G and rs4778889 T/C polymorphisms on the altered risk of CRC.
Authors: Hongmei Nan; Carolyn M Hutter; Yi Lin; Eric J Jacobs; Cornelia M Ulrich; Emily White; John A Baron; Sonja I Berndt; Hermann Brenner; Katja Butterbach; Bette J Caan; Peter T Campbell; Christopher S Carlson; Graham Casey; Jenny Chang-Claude; Stephen J Chanock; Michelle Cotterchio; David Duggan; Jane C Figueiredo; Charles S Fuchs; Edward L Giovannucci; Jian Gong; Robert W Haile; Tabitha A Harrison; Richard B Hayes; Michael Hoffmeister; John L Hopper; Thomas J Hudson; Mark A Jenkins; Shuo Jiao; Noralane M Lindor; Mathieu Lemire; Loic Le Marchand; Polly A Newcomb; Shuji Ogino; Bethann M Pflugeisen; John D Potter; Conghui Qu; Stephanie A Rosse; Anja Rudolph; Robert E Schoen; Fredrick R Schumacher; Daniela Seminara; Martha L Slattery; Stephen N Thibodeau; Fridtjof Thomas; Mark Thornquist; Greg S Warnick; Brent W Zanke; W James Gauderman; Ulrike Peters; Li Hsu; Andrew T Chan Journal: JAMA Date: 2015-03-17 Impact factor: 56.272