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Literature DB >> 22183598

Reconstitution of modified chromatin templates for in vitro functional assays.

Miyong Yun¹, Chun Ruan, Jae-Wan Huh, Bing Li.

Abstract

To study the functions of histone modifications in the context of chromatin, it is necessary to be able to prepare nucleosomal templates that carry specific posttranslational modifications in a defined biochemical system. Here, we describe two sets of protocols for reconstituting designer nucleosomes that contain specifically modified histones. The resulting nucleosomes are suitable for electromobility shift assays, chromatin remodeling assays, and other functional and structural studies.

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Year: 2012 PMID： 22183598 PMCID： PMC3749000 DOI： 10.1007/978-1-61779-477-3_15

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

13 in total

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13 in total

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