Literature DB >> 25827872

Preparation and analysis of positioned mononucleosomes.

Olga I Kulaeva1, Vasily M Studitsky.   

Abstract

Short DNA fragments containing single nucleosomes have been extensively employed as simple model experimental systems for analysis of many intranuclear processes, including binding of proteins to nucleosomes, covalent histone modifications, transcription, DNA repair, and ATP-dependent chromatin remodeling. Here we describe several recently developed procedures for obtaining and analysis of mononucleosomes assembled on 200-350-bp DNA fragments.

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Year:  2015        PMID: 25827872      PMCID: PMC4966548          DOI: 10.1007/978-1-4939-2474-5_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  38 in total

1.  Preparation of nucleosome core particle from recombinant histones.

Authors:  K Luger; T J Rechsteiner; T J Richmond
Journal:  Methods Enzymol       Date:  1999       Impact factor: 1.600

Review 2.  Chromatin remodeling by RNA polymerases.

Authors:  Vasily M Studitsky; Wendy Walter; Maria Kireeva; Mikhail Kashlev; Gary Felsenfeld
Journal:  Trends Biochem Sci       Date:  2004-03       Impact factor: 13.807

3.  Transcription through the nucleosome by mRNA-producing RNA polymerases.

Authors:  W Walter; M Kashlev; V M Studitsky
Journal:  Methods Enzymol       Date:  2004       Impact factor: 1.600

4.  Preparation of defined mononucleosomes, dinucleosomes, and nucleosome arrays in vitro and analysis of transcription factor binding.

Authors:  Lisa Ann Cirillo; Kenneth S Zaret
Journal:  Methods Enzymol       Date:  2004       Impact factor: 1.600

5.  Approaches for studying nucleosome movement by ATP-dependent chromatin remodeling complexes.

Authors:  Swetansu K Hota; Blaine Bartholomew
Journal:  Methods Mol Biol       Date:  2012

6.  Transcriptional activation triggers deposition and removal of the histone variant H3.3.

Authors:  Brian E Schwartz; Kami Ahmad
Journal:  Genes Dev       Date:  2005-03-17       Impact factor: 11.361

7.  Crystal structure of the nucleosome core particle at 2.8 A resolution.

Authors:  K Luger; A W Mäder; R K Richmond; D F Sargent; T J Richmond
Journal:  Nature       Date:  1997-09-18       Impact factor: 49.962

8.  Use of selectively trypsinized nucleosome core particles to analyze the role of the histone "tails" in the stabilization of the nucleosome.

Authors:  J Ausio; F Dong; K E van Holde
Journal:  J Mol Biol       Date:  1989-04-05       Impact factor: 5.469

9.  Nucleosomal H2B ubiquitylation with purified factors.

Authors:  Jaehoon Kim; Robert G Roeder
Journal:  Methods       Date:  2011-04-03       Impact factor: 3.608

10.  Dynamics of replication-independent histone turnover in budding yeast.

Authors:  Michael F Dion; Tommy Kaplan; Minkyu Kim; Stephen Buratowski; Nir Friedman; Oliver J Rando
Journal:  Science       Date:  2007-03-09       Impact factor: 47.728

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  1 in total

1.  Structure of transcribed chromatin is a sensor of DNA damage.

Authors:  Nikolay A Pestov; Nadezhda S Gerasimova; Olga I Kulaeva; Vasily M Studitsky
Journal:  Sci Adv       Date:  2015-07-03       Impact factor: 14.136

  1 in total

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