| Literature DB >> 22183591 |
Ashwin Unnikrishnan1, Bungo Akiyoshi, Sue Biggins, Toshio Tsukiyama.
Abstract
We have recently established a system for purifying minichromosomes in a native state from Saccharomyces cerevisiae. This system is extremely efficient, and a single-step purification yields samples with sufficient purity and quantity for mass spectrometry (MS) analysis of histones and non-histone proteins tightly associated with the minichromosome. The templates can also be used in various biochemical assays in vitro, such as transcription and recombination, and could be suitable for EM or other biophysical studies.Entities:
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Year: 2012 PMID： 22183591 PMCID： PMC3243952 DOI： 10.1007/978-1-61779-477-3_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745