PURPOSES: Outcomes following lung transplantation are limited by bronchiolitis obliterans syndrome (BOS). As the number of circulating regulatory T cells (Treg) is lower in lung recipients with BOS than in stable lung recipients, we hypothesized that Treg is also correlated with lung function in the early post-transplantation period. METHODS: This prospective study included 18 consecutive patients whose lung function parameters were recorded 3 weeks and 3 months after transplantation, between February and July 2007. Peripheral blood mononuclear cells were stained with anti-CD3, -CD4, -CD8, -CD19, -CD25, -CD28, -CD45RA, -CD45RO, -CD69, -CD127, -CTLA4, and -Foxp3 antibodies and FACS assays were performed. In addition, intracellular cytokines were stained for FACS. RESULTS: Treg-specific markers (Foxp3, CD127(lo), and CTLA4) in the CD25+ CD4+ population were correlated with both forced expiratory volume in 1 s and forced vital capacity. Th1-cytokine secretion was more dominant in CD4+ CD25+ T cells than in CD4+ CD25- T cells. In contrast, Th2 and Treg cytokine secretion was the dominant response in stable recipients. CONCLUSIONS: The frequency of Treg cells was positively correlated with good lung function in the early period after lung transplantation.
PURPOSES: Outcomes following lung transplantation are limited by bronchiolitis obliterans syndrome (BOS). As the number of circulating regulatory T cells (Treg) is lower in lung recipients with BOS than in stable lung recipients, we hypothesized that Treg is also correlated with lung function in the early post-transplantation period. METHODS: This prospective study included 18 consecutive patients whose lung function parameters were recorded 3 weeks and 3 months after transplantation, between February and July 2007. Peripheral blood mononuclear cells were stained with anti-CD3, -CD4, -CD8, -CD19, -CD25, -CD28, -CD45RA, -CD45RO, -CD69, -CD127, -CTLA4, and -Foxp3 antibodies and FACS assays were performed. In addition, intracellular cytokines were stained for FACS. RESULTS: Treg-specific markers (Foxp3, CD127(lo), and CTLA4) in the CD25+ CD4+ population were correlated with both forced expiratory volume in 1 s and forced vital capacity. Th1-cytokine secretion was more dominant in CD4+ CD25+ T cells than in CD4+ CD25- T cells. In contrast, Th2 and Treg cytokine secretion was the dominant response in stable recipients. CONCLUSIONS: The frequency of Treg cells was positively correlated with good lung function in the early period after lung transplantation.
Authors: Elbert P Trulock; Leah B Edwards; David O Taylor; Mark M Boucek; Berkeley M Keck; Marshall I Hertz Journal: J Heart Lung Transplant Date: 2004-07 Impact factor: 10.247
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Authors: Weihong Liu; Amy L Putnam; Zhou Xu-Yu; Gregory L Szot; Michael R Lee; Shirley Zhu; Peter A Gottlieb; Philipp Kapranov; Thomas R Gingeras; Barbara Fazekas de St Groth; Carol Clayberger; David M Soper; Steven F Ziegler; Jeffrey A Bluestone Journal: J Exp Med Date: 2006-07-03 Impact factor: 14.307
Authors: J M Diamond; E Cantu; M K Porteous; Y Suzuki; K C Meyer; D J Lederer; R K Milewski; S Arcasoy; F D'Ovidio; M Bacchetta; J R Sonett; G Singh; J Costa; J W Tobias; H Rodriguez; V M Van Deerlin; K M Olthoff; A Shaked; B-L Chang; J D Christie Journal: Am J Transplant Date: 2017-02-17 Impact factor: 8.086
Authors: John R Greenland; Charissa M Wong; Rahul Ahuja; Angelia S Wang; Chiyo Uchida; Jeffrey A Golden; Steven R Hays; Lorriana E Leard; Raja Rajalingam; Jonathan P Singer; Jasleen Kukreja; Paul J Wolters; George H Caughey; Qizhi Tang Journal: Transplantation Date: 2016-10 Impact factor: 4.939