BACKGROUND: The subset of CD4+CD25+ regulatory T cells, recently identified in humans, may play a central role in the regulation of immune tolerance to graft survival. METHODS: This study assesses the frequency and functional profile of CD4+CD25+CD69- cells in the peripheral blood of lung transplant recipients (>3 years from transplantation), 10 of whom were in a stable clinical condition and 11 of whom demonstrated chronic rejection (bronchiolitis obliterans syndrome). We also studied a group of seven healthy subjects. RESULTS: The frequency of CD4+ T cells expressing CD25 (CD4+CD25+) and the highest levels (CD25) were lower in patients with bronchiolitis obliterans syndrome compared with healthy subjects and subjects in a stable clinical condition (P < or = 0.01). Purified CD4+CD25+ cells exhibited a regulatory functional profile in vitro: they were hyporesponsive, suppressed the proliferation of CD4+CD25- cells, and produced interleukin-10. CONCLUSION: These results provide in vivo evidence that peripheral CD4+CD25+ T cells may represent an important regulatory subset in lung transplantation.
BACKGROUND: The subset of CD4+CD25+ regulatory T cells, recently identified in humans, may play a central role in the regulation of immune tolerance to graft survival. METHODS: This study assesses the frequency and functional profile of CD4+CD25+CD69- cells in the peripheral blood of lung transplant recipients (>3 years from transplantation), 10 of whom were in a stable clinical condition and 11 of whom demonstrated chronic rejection (bronchiolitis obliterans syndrome). We also studied a group of seven healthy subjects. RESULTS: The frequency of CD4+ T cells expressing CD25 (CD4+CD25+) and the highest levels (CD25) were lower in patients with bronchiolitis obliterans syndrome compared with healthy subjects and subjects in a stable clinical condition (P < or = 0.01). Purified CD4+CD25+ cells exhibited a regulatory functional profile in vitro: they were hyporesponsive, suppressed the proliferation of CD4+CD25- cells, and produced interleukin-10. CONCLUSION: These results provide in vivo evidence that peripheral CD4+CD25+ T cells may represent an important regulatory subset in lung transplantation.
Authors: Tomoyuki Nakagiri; Gregor Warnecke; Murat Avsar; Stefanie Thissen; Bianca Kruse; Christian Kühn; Petra Ziehme; Ann-Kathrin Knöfel; Nodir Madrahimov; Meinoshin Okumura; Yoshiki Sawa; Jens Gottlieb; André R Simon; Axel Haverich; Martin Strüber Journal: Surg Today Date: 2011-12-17 Impact factor: 2.549
Authors: K L Pothoven; T Kheradmand; Q Yang; J L Houlihan; H Zhang; M Degutes; S D Miller; X Luo Journal: Am J Transplant Date: 2010-07-12 Impact factor: 8.086
Authors: David C Neujahr; Adriana C Cardona; Onome Ulukpo; Mark Rigby; Andres Pelaez; Allan Ramirez; Anthony A Gal; Seth D Force; E Clinton Lawrence; Allan D Kirk; Christian P Larsen Journal: Transplantation Date: 2009-08-27 Impact factor: 4.939