RATIONALE AND OBJECTIVES: To evaluate cone beam computed tomography (CBCT) for monitoring of tibial bone defect healing in comparison to histopathological findings. MATERIALS AND METHODS: Circumscribed tibial bone defects were created in 16 mini-pigs and imaging of the tibia was performed on day 42 using a modern CBCT scanner with flat panel detector (PaX-Duo3D, Vatech, Korea). The extent of osseous consolidation including remaining calcium phosphate granules was measured quantitatively by a CBCT volumetry tool using commercially available software (Osirix Imaging software, Pixmeo, Geneva, Switzerland). Volumes of the entire defect (including all pixels), areas of osseous consolidation (density values >2350) and nonmineralized areas (density values <2350) of the defect were determined. The extent of bone regeneration was determined and correlated with the histomorphometrical reference standard. Independently, a visual semiquantitative CBCT-score was applied (4-point scale) to assess bone defect healing. RESULTS: The extent of osseous consolidation in CBCT volumetry ranged from 14% to 92% (mean, 63.4 ± 17.6%). There was a significant positive correlation between histologically visible newly formed bone and the extent of bone regeneration on CBCT volumetry (r = 0.74-0.79, P < .001). The visual score matched with the volumetric results in 75% of the cases. CONCLUSION: CBCT volumetry allows for reliable, noninvasive quantitative monitoring of bone defect healing and correlates significantly with histological findings. CBCT is a promising technique for imaging of peripheral bones suggesting further evaluation in clinical trials.
RATIONALE AND OBJECTIVES: To evaluate cone beam computed tomography (CBCT) for monitoring of tibial bone defect healing in comparison to histopathological findings. MATERIALS AND METHODS: Circumscribed tibial bone defects were created in 16 mini-pigs and imaging of the tibia was performed on day 42 using a modern CBCT scanner with flat panel detector (PaX-Duo3D, Vatech, Korea). The extent of osseous consolidation including remaining calcium phosphate granules was measured quantitatively by a CBCT volumetry tool using commercially available software (Osirix Imaging software, Pixmeo, Geneva, Switzerland). Volumes of the entire defect (including all pixels), areas of osseous consolidation (density values >2350) and nonmineralized areas (density values <2350) of the defect were determined. The extent of bone regeneration was determined and correlated with the histomorphometrical reference standard. Independently, a visual semiquantitative CBCT-score was applied (4-point scale) to assess bone defect healing. RESULTS: The extent of osseous consolidation in CBCT volumetry ranged from 14% to 92% (mean, 63.4 ± 17.6%). There was a significant positive correlation between histologically visible newly formed bone and the extent of bone regeneration on CBCT volumetry (r = 0.74-0.79, P < .001). The visual score matched with the volumetric results in 75% of the cases. CONCLUSION: CBCT volumetry allows for reliable, noninvasive quantitative monitoring of bone defect healing and correlates significantly with histological findings. CBCT is a promising technique for imaging of peripheral bones suggesting further evaluation in clinical trials.
Authors: J P Grassmann; J Schneppendahl; M Sager; A R Hakimi; M Herten; T T Loegters; M Wild; M Hakimi; J Windolf; P Jungbluth Journal: J Mater Sci Mater Med Date: 2015-01-11 Impact factor: 3.896
Authors: Greg M Osgood; Gaurav K Thawait; Nima Hafezi-Nejad; Delaram Shakoor; Adam Shaner; John Yorkston; Wojciech B Zbijewski; Jeffrey H Siewerdsen; Shadpour Demehri Journal: Br J Radiol Date: 2017-03-10 Impact factor: 3.039
Authors: Pascal Jungbluth; Lucas-Sebastian Spitzhorn; Jan Grassmann; Stephan Tanner; David Latz; Md Shaifur Rahman; Martina Bohndorf; Wasco Wruck; Martin Sager; Vera Grotheer; Patric Kröpil; Mohssen Hakimi; Joachim Windolf; Johannes Schneppendahl; James Adjaye Journal: Bone Res Date: 2019-10-24 Impact factor: 13.567