Literature DB >> 22162553

Multiplex real-time PCR assay and melting curve analysis for identifying Mycobacterium tuberculosis complex and nontuberculous mycobacteria.

Jeong-Uk Kim1, Choong-Hwan Cha, Hae-Kyong An.   

Abstract

A multiplex real-time PCR assay and melting curve analysis for identifying 23 mycobacterial species was developed and evaluated using 77 reference strains and 369 clinical isolates. Concordant results were obtained for all 189 (100%) isolates of the Mycobacterium tuberculosis complex and 169 (93.9%) isolates of nontuberculous mycobacteria. Our results showed that this multiplex real-time PCR assay is an effective tool for the mycobacterial identification from cultures.

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Year:  2011        PMID： 22162553      PMCID： PMC3264165          DOI： 10.1128/JCM.06155-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  31 in total

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Authors:  S Shenai; C Rodrigues; A Mehta
Journal:  Int J Tuberc Lung Dis       Date:  2010-08       Impact factor: 2.373

2.  Use of the GenoType Mycobacterium CM and AS assays to analyze 76 nontuberculous mycobacterial isolates from Greece.

Authors:  Zoe Gitti; Ioannis Neonakis; Garyfallia Fanti; Fanourios Kontos; Sofia Maraki; Yiannis Tselentis
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3.  Intuitive hypertext-based molecular identification of micro-organisms.

Authors:  D Harmsen; J Rothgänger; C Singer; J Albert; M Frosch
Journal:  Lancet       Date:  1999-01-23       Impact factor: 79.321

4.  Differentiation of mycobacterial species by PCR-restriction analysis of DNA (342 base pairs) of the RNA polymerase gene (rpoB).

Authors:  B J Kim; K H Lee; B N Park; S J Kim; G H Bai; S J Kim; Y H Kook
Journal:  J Clin Microbiol       Date:  2001-06       Impact factor: 5.948

5.  Development and application of multiprobe real-time PCR method targeting the hsp65 gene for differentiation of Mycobacterium species from isolates and sputum specimens.

Authors:  Kijeong Kim; Hyungki Lee; Mi-Kyung Lee; Seoung-Ae Lee; Tae-Sun Shim; Seong Yong Lim; Won-Jung Koh; Jae-Joon Yim; Bazarragchaa Munkhtsetseg; Wonyong Kim; Sang-In Chung; Yoon-Hoh Kook; Bum-Joon Kim
Journal:  J Clin Microbiol       Date:  2010-07-14       Impact factor: 5.948

6.  Diagnostic application of genotypic identification of mycobacteria.

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Journal:  J Med Microbiol       Date:  2006-05       Impact factor: 2.472

Review 7.  Non-tuberculous mycobacteria in TB-endemic countries: are we neglecting the danger?

Authors:  Krishnamoorthy Gopinath; Sarman Singh
Journal:  PLoS Negl Trop Dis       Date:  2010-04-27

8.  Development of a simple and low-cost real-time PCR method for the identification of commonly encountered mycobacteria in a high throughput laboratory.

Authors:  K L Leung; C W Yip; W F Cheung; A C T Lo; W M Ko; K M Kam
Journal:  J Appl Microbiol       Date:  2009-04-22       Impact factor: 3.772

9.  Clinical significance of nontuberculous mycobacteria isolated from respiratory specimens in Korea.

Authors:  Won-Jung Koh; O Jung Kwon; Kyeongman Jeon; Tae Sung Kim; Kyung Soo Lee; Young Kil Park; Gill Han Bai
Journal:  Chest       Date:  2006-02       Impact factor: 9.410

Review 10.  Pulmonary disease due to nontuberculous mycobacteria.

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Journal:  Chest       Date:  2008-01       Impact factor: 9.410
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  8 in total

1.  Evaluation of performance of the Real-Q NTM-ID kit for rapid identification of eight nontuberculous mycobacterial species.

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Journal:  J Clin Microbiol       Date:  2014-08-27       Impact factor: 5.948

2.  Development of a One-Step Multiplex PCR Assay for Differential Detection of Major Mycobacterium Species.

Authors:  Hansong Chae; Seung Jung Han; Su-Young Kim; Chang-Seok Ki; Hee Jae Huh; Dongeun Yong; Won-Jung Koh; Sung Jae Shin
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3.  Rapid inactivation of Mycobacterium and nocardia species before identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Authors:  W Michael Dunne; Kirk Doing; Elizabeth Miller; Eric Miller; Erik Moreno; Mehdi Baghli; Sandrine Mailler; Victoria Girard; Alex van Belkum; Parampal Deol
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4.  Drug Susceptibility Testing of 31 Antimicrobial Agents on Rapidly Growing Mycobacteria Isolates from China.

Authors:  Hui Pang; Guilian Li; Xiuqin Zhao; Haican Liu; Kanglin Wan; Ping Yu
Journal:  Biomed Res Int       Date:  2015-08-13       Impact factor: 3.411

5.  A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses.

Authors:  Zhenzhou Wan; Ya'nan Zhang; Zhixiang He; Jia Liu; Ke Lan; Yihong Hu; Chiyu Zhang
Journal:  Int J Mol Sci       Date:  2016-11-23       Impact factor: 5.923

6.  Single-nucleotide polymorphisms related to fluoroquinolone and aminoglycoside resistance in Mycobacterium avium isolates.

Authors:  Hui Pang; Kanglin Wan; Lin Wei
Journal:  Infect Drug Resist       Date:  2018-04-09       Impact factor: 4.003

7.  Added diagnostic value of 16S rRNA gene pan-mycobacterial PCR for nontuberculous mycobacterial infections: a 10-year retrospective study.

Authors:  Andenmatten Simon; Opota Onya; Jesica Mazza-Stalder; Laurent Nicod; Greub Gilbert; Jaton Katia
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2019-07-16       Impact factor: 3.267

8.  Identification of nontuberculous mycobacteria species by multiplex real-time PCR with high-resolution melting.

Authors:  Aline Dos Santos Peixoto; Lílian Maria Lapa Montenegro; Andrea Santos Lima; Fábio Lopes Melo; Walter Lins Barbosa Júnior; Maria Madileuza Carneiro Neves; Jesus Pais Ramos; Haiana Charifker Schindler; Zulma Maria Medeiros
Journal:  Rev Soc Bras Med Trop       Date:  2020-11-06       Impact factor: 1.581
  8 in total

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