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Literature DB >> 25165078

Evaluation of performance of the Real-Q NTM-ID kit for rapid identification of eight nontuberculous mycobacterial species.

Hee Jae Huh¹, Kyung Sun Park¹, Mi-Ae Jang¹, Ji-Youn Kim², Hyeon Jeong Kwon², Chang-Seok Ki³, Nam Yong Lee³.

Abstract

We evaluated a multiplex real-time PCR and melting curve analysis assay (Real-Q NTM-ID kit; Biosewoom, Seoul, South Korea) for the identification of eight common nontuberculous mycobacterial species, using 30 type strains and 230 consecutive clinical isolates. The concordance rate of this assay with multigene sequence-based typing was 97.0% (223/230 isolates).

Entities: Gene

Mesh：

Substances：
Reagent Kits, Diagnostic

Year: 2014 PMID： 25165078 PMCID： PMC4313269 DOI： 10.1128/JCM.01957-14

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

15 in total

1. Sequence-based identification of Mycobacterium species using the MicroSeq 500 16S rDNA bacterial identification system.

Authors: J B Patel; D G Leonard; X Pan; J M Musser; R E Berman; I Nachamkin
Journal: J Clin Microbiol Date: 2000-01 Impact factor: 5.948

2. Evaluation of genotype and LiPA MYCOBACTERIA assays for identification of Finnish mycobacterial isolates.

Authors: Johanna Mäkinen; Aleksi Sarkola; Merja Marjamäki; Matti K Viljanen; Hanna Soini
Journal: J Clin Microbiol Date: 2002-09 Impact factor: 5.948

Review 3. An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases.

Authors: David E Griffith; Timothy Aksamit; Barbara A Brown-Elliott; Antonino Catanzaro; Charles Daley; Fred Gordin; Steven M Holland; Robert Horsburgh; Gwen Huitt; Michael F Iademarco; Michael Iseman; Kenneth Olivier; Stephen Ruoss; C Fordham von Reyn; Richard J Wallace; Kevin Winthrop
Journal: Am J Respir Crit Care Med Date: 2007-02-15 Impact factor: 21.405

4. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis.

Authors: A Telenti; F Marchesi; M Balz; F Bally; E C Böttger; T Bodmer
Journal: J Clin Microbiol Date: 1993-02 Impact factor: 5.948

5. Development and application of multiprobe real-time PCR method targeting the hsp65 gene for differentiation of Mycobacterium species from isolates and sputum specimens.

Authors: Kijeong Kim; Hyungki Lee; Mi-Kyung Lee; Seoung-Ae Lee; Tae-Sun Shim; Seong Yong Lim; Won-Jung Koh; Jae-Joon Yim; Bazarragchaa Munkhtsetseg; Wonyong Kim; Sang-In Chung; Yoon-Hoh Kook; Bum-Joon Kim
Journal: J Clin Microbiol Date: 2010-07-14 Impact factor: 5.948

Evaluation of performance of the Real-Q NTM-ID kit for rapid identification of eight nontuberculous mycobacterial species.

1. Sequence-based identification of Mycobacterium species using the MicroSeq 500 16S rDNA bacterial identification system.

2. Evaluation of genotype and LiPA MYCOBACTERIA assays for identification of Finnish mycobacterial isolates.

Review 3. An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases.

4. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis.

5. Development and application of multiprobe real-time PCR method targeting the hsp65 gene for differentiation of Mycobacterium species from isolates and sputum specimens.

6. Diagnostic application of genotypic identification of mycobacteria.

7. Sequence-based differentiation of strains in the Mycobacterium avium complex.

Review 8. Nontuberculous mycobacterial lung disease.

9. Clinical significance of nontuberculous mycobacteria isolated from respiratory specimens in Korea.

Review 10. Update on the epidemiology of pulmonary nontuberculous mycobacterial infections.

1. Development of a One-Step Multiplex PCR Assay for Differential Detection of Major Mycobacterium Species.

Review 2. Pulmonary non-tuberculous mycobacterial infections: current state and future management.