BACKGROUND: The aim of this study was to assess whether the quantities of factor VIII and factor IX in fresh-frozen plasma produced from whole blood stored at 4 °C for 24 hours are adequate for their intended purpose. MATERIALS AND METHODS: Fresh-frozen plasma separated from whole blood after storage at 4 °C overnight (24 hours from donation) was compared with plasma prepared 8 hours after donation using a standard method. The amounts of factor VIII and factor IX obtained with the two methods were compared. RESULTS: Compared to the levels of factor VIII and factor IX in plasma prepared within 8 hours of blood collection, the levels in plasma prepared after 24 hours of storage at 4 °C were 25% and 9% lower, respectively. Ninety percent of the factor VIII and 100% of the factor IX levels were above 0.5 IU/mL (standard haematology reference range) after 24 hours of storage. DISCUSSION: These data suggest that there is good retention of coagulation factor activity in plasma produced from whole blood stored at 4 ºC for 24 hours and that such plasma would be an acceptable product for most patients requiring fresh-frozen plasma.
BACKGROUND: The aim of this study was to assess whether the quantities of factor VIII and factor IX in fresh-frozen plasma produced from whole blood stored at 4 °C for 24 hours are adequate for their intended purpose. MATERIALS AND METHODS: Fresh-frozen plasma separated from whole blood after storage at 4 °C overnight (24 hours from donation) was compared with plasma prepared 8 hours after donation using a standard method. The amounts of factor VIII and factor IX obtained with the two methods were compared. RESULTS: Compared to the levels of factor VIII and factor IX in plasma prepared within 8 hours of blood collection, the levels in plasma prepared after 24 hours of storage at 4 °C were 25% and 9% lower, respectively. Ninety percent of the factor VIII and 100% of the factor IX levels were above 0.5 IU/mL (standard haematology reference range) after 24 hours of storage. DISCUSSION: These data suggest that there is good retention of coagulation factor activity in plasma produced from whole blood stored at 4 ºC for 24 hours and that such plasma would be an acceptable product for most patients requiring fresh-frozen plasma.
Authors: R Cardigan; P F Van der Meer; C Pergande; P Cookson; B Baumann-Baretti; J A Cancelas; D Devine; H Gulliksson; R Vassallo; J de Wildt-Eggen Journal: Transfusion Date: 2011-01 Impact factor: 3.157
Authors: D F O'Shaughnessy; C Atterbury; P Bolton Maggs; M Murphy; D Thomas; S Yates; L M Williamson Journal: Br J Haematol Date: 2004-07 Impact factor: 6.998
Authors: Faisal A Al-Allaf; Mohiuddin M Taher; Zainularifeen Abduljaleel; Abdellatif Bouazzaoui; Mohammed Athar; Neda M Bogari; Halah A Abalkhail; Tarek Ma Owaidah Journal: J Clin Med Res Date: 2017-02-21