| Literature DB >> 22144271 |
Jared Johnson1, Richard A Cerione, Jon W Erickson.
Abstract
Tight regulation of Rho GTPase-signaling functions requires the proper localization of proteins to the membrane and cytosolic compartments, which can themselves undergo reconfiguration in response to signaling events. The importance of lipid-mediated membrane signal transduction continues to emerge as a critical event in many Rho GTPase-signaling pathways. Here we describe methods for the reconstitution of lipid-modified Rho GTPases with defined lipid vesicles and how this system can be used as a real-time assay for monitoring protein-membrane interactions.Entities:
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Year: 2012 PMID： 22144271 PMCID： PMC3632373 DOI： 10.1007/978-1-61779-442-1_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745