Literature DB >> 22139181

Crystallization and X-ray data collection of HP0902 from Helicobacter pylori 26695.

Dae Won Sim1, Jung Hyun Song, Woo Cheol Lee, Yoo Sup Lee, Hye Yeon Kim, Hyung Sik Won.   

Abstract

HP0902 from Helicobacter pylori 26695 belongs to the cupin superfamily of proteins, which encompasses proteins with a great diversity in function. In this work, two types of recombinant HP0902 protein were crystallized: one with an N-terminal His(6) tag ((H6)HP0902) and the other with a C-terminal His(6) tag (HP0902(H6)). The (H6)HP0902 crystal diffracted to 1.40 Å resolution and belonged to space group P2(1), with unit-cell parameters a = 33.5, b = 78.6, c = 41.4 Å. The HP0902(H6) crystal belonged to space group P4(3)2(1)2 or P4(1)2(1)2 and diffracted to 2.5 Å resolution, with unit-cell parameters a = b = 50.4, c = 142.0 Å.

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Year:  2011        PMID: 22139181      PMCID: PMC3232154          DOI: 10.1107/S1744309111039315

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN: 1744-3091


  12 in total

Review 1.  Cupins: the most functionally diverse protein superfamily?

Authors:  Jim M Dunwell; Alan Purvis; Sawsan Khuri
Journal:  Phytochemistry       Date:  2004-01       Impact factor: 4.072

Review 2.  Allergens of the cupin superfamily.

Authors:  E N Mills; J Jenkins; N Marigheto; P S Belton; A P Gunning; V J Morris
Journal:  Biochem Soc Trans       Date:  2002-11       Impact factor: 5.407

3.  HP0902 from Helicobacter pylori is a thermostable, dimeric protein belonging to an all-beta topology of the cupin superfamily.

Authors:  Dae-Won Sim; Yoo-Sup Lee; Ji-Hun Kim; Min-Duk Seo; Bong-Jin Lee; Hyung-Sik Won
Journal:  BMB Rep       Date:  2009-06-30       Impact factor: 4.778

4.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

5.  The fdxA ferredoxin gene can down-regulate frxA nitroreductase gene expression and is essential in many strains of Helicobacter pylori.

Authors:  Asish K Mukhopadhyay; Jin-Yong Jeong; Daiva Dailidiene; Paul S Hoffman; Douglas E Berg
Journal:  J Bacteriol       Date:  2003-05       Impact factor: 3.490

6.  The Pfam protein families database.

Authors:  Robert D Finn; Jaina Mistry; John Tate; Penny Coggill; Andreas Heger; Joanne E Pollington; O Luke Gavin; Prasad Gunasekaran; Goran Ceric; Kristoffer Forslund; Liisa Holm; Erik L L Sonnhammer; Sean R Eddy; Alex Bateman
Journal:  Nucleic Acids Res       Date:  2009-11-17       Impact factor: 16.971

Review 7.  Pleiotropic actions of Helicobacter pylori vacuolating cytotoxin, VacA.

Authors:  Hajime Isomoto; Joel Moss; Toshiya Hirayama
Journal:  Tohoku J Exp Med       Date:  2010-01       Impact factor: 1.848

8.  Proteins released by Helicobacter pylori in vitro.

Authors:  Nayoung Kim; David L Weeks; Jai Moo Shin; David R Scott; Mary K Young; George Sachs
Journal:  J Bacteriol       Date:  2002-11       Impact factor: 3.490

Review 9.  Scaling and assessment of data quality.

Authors:  Philip Evans
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-12-14

10.  Overview of the CCP4 suite and current developments.

Authors:  Martyn D Winn; Charles C Ballard; Kevin D Cowtan; Eleanor J Dodson; Paul Emsley; Phil R Evans; Ronan M Keegan; Eugene B Krissinel; Andrew G W Leslie; Airlie McCoy; Stuart J McNicholas; Garib N Murshudov; Navraj S Pannu; Elizabeth A Potterton; Harold R Powell; Randy J Read; Alexei Vagin; Keith S Wilson
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2011-03-18
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