| Literature DB >> 22138018 |
Yan Fen Ma1, Louis M Weiss, Huan Huang.
Abstract
Analysis of gene function in Trypanosoma cruzi is limited due to the absence of rapid, simple and reversible genetic tools to regulate gene and corresponding protein expression. We have designed a modified pTREX vector which uses an N-terminal fusion of a ligand-controlled destabilisation domain (ddFKBP) to a gene/protein of interest. This vector allows rapid and reversible protein expression and efficient functional analysis of proteins in different T. cruzi life cycle stages.Entities:
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Year: 2011 PMID： 22138018 PMCID： PMC3249466 DOI： 10.1016/j.ijpara.2011.11.002
Source DB: PubMed Journal: Int J Parasitol ISSN： 0020-7519 Impact factor: 3.981