PURPOSE: To assess follicular growth after xenografting in order to understand how freezing and/or grafting may affect follicular development. METHODS: Human ovarian biopsies were used for fresh and frozen-thawed xenografting to SCID mice. After xenotransplantation, follicular morphology and proportion, oocyte and follicle diameter, and quantitative and qualitative parameters of antral follicles were analyzed. RESULTS: The proportion of growing follicles was significantly higher in grafted than non-grafted ovarian tissue. Follicular growth to the antral stage was observed and there was no significant difference in oocyte or follicle diameter in fresh or frozen-thawed grafts. Although no significant difference was observed in antral area or zona pellucida thickness, the theca layer in antral follicles from frozen-thawed grafted tissue was found to be significantly thinner than in fresh grafts. CONCLUSION: Antral follicles obtained after grafting of frozen-thawed human ovarian tissue showed a thinner theca cell layer compared to those from fresh grafts, which could affect follicular development and function. Further studies are nevertheless warranted to confirm the identity of theca cells and assess if they retain the ability to respond to luteinizing hormone and produce androgens.
PURPOSE: To assess follicular growth after xenografting in order to understand how freezing and/or grafting may affect follicular development. METHODS:Human ovarian biopsies were used for fresh and frozen-thawed xenografting to SCIDmice. After xenotransplantation, follicular morphology and proportion, oocyte and follicle diameter, and quantitative and qualitative parameters of antral follicles were analyzed. RESULTS: The proportion of growing follicles was significantly higher in grafted than non-grafted ovarian tissue. Follicular growth to the antral stage was observed and there was no significant difference in oocyte or follicle diameter in fresh or frozen-thawed grafts. Although no significant difference was observed in antral area or zona pellucida thickness, the theca layer in antral follicles from frozen-thawed grafted tissue was found to be significantly thinner than in fresh grafts. CONCLUSION: Antral follicles obtained after grafting of frozen-thawed human ovarian tissue showed a thinner theca cell layer compared to those from fresh grafts, which could affect follicular development and function. Further studies are nevertheless warranted to confirm the identity of theca cells and assess if they retain the ability to respond to luteinizing hormone and produce androgens.
Authors: M M Dolmans; M M Binda; S Jacobs; J P Dehoux; J L Squifflet; J Ambroise; J Donnez; C A Amorim Journal: J Assist Reprod Genet Date: 2015-08-04 Impact factor: 3.412
Authors: Fariba Khosravi; Robert L Reid; Ashraf Moini; Farid Abolhassani; Mojtaba R Valojerdi; Frederick W K Kan Journal: J Assist Reprod Genet Date: 2013-10-25 Impact factor: 3.412
Authors: Limor Man; Laura Park; Richard Bodine; Michael Ginsberg; Nikica Zaninovic; Glenn Schattman; Robert E Schwartz; Zev Rosenwaks; Daylon James Journal: J Vis Exp Date: 2018-05-16 Impact factor: 1.355